L-半胱氨酸对冻融后猪精子质量参数和膜脂质过氧化反应的影响

试验检测冷冻稀释液中添加L-半胱氨酸对冻精解冻后精子质量参数、膜脂质过氧化程度的影响。结果显示:(1)在冷冻稀释液中添加0.1 mg.mL-1L-半胱氨酸能显著地提高冻融后精子的活率、顶体完整率及体外受精率(P<0.05),但对运动学参数没有影响(P>0.05)。(2)在冷冻稀释液中添加L-半胱氨酸(0.1、0.2和0.3mg.mL-1L-半胱氨酸)能显著降低膜脂质过氧化水平(P<0.05)。该结果表明冷冻稀释液中添加L-半胱氨酸能改善猪精子的冷冻效率。

Effects of addition of L-cysteine on quality parameters and membrane lipid peroxidation of frozen-thawed boar sperm

The main objective of this study was to examine the effects of addition of L-cysteine in diluents on the qualitative parameters and peroxidation level of membrane lipid of frozen-thawed sperm.The results show that:(1)Addition of 0.1 mg·mL~(-1) L-cysteine in diluents can significantly improve the viability ,the rate of acrosomal integrity and in vitro fertilization rate of frozen-thawed sperm (P<0.05),but does not affect the kinematic parameters (P>0.05).(2)Addition of L-cysteine (0.1,0.2 and 0.3 mg·mL~(-1) L-cysteine) in diluents can significantly reduce the level of membrane lipid peroxidation (P<0.05).Our results show that addition of L-cysteine in diluents can improve the efficiency of pig sperm cryopreservation.