| 长豇豆AFLP技术体系的构建与优化 |
| |
| 引用本文： | 刘永华,李国景,吴晓花,汪宝根,鲁忠富,朱祝军.长豇豆AFLP技术体系的构建与优化[J].浙江农业学报,2007,19(3):156-159. |
| |
| 作者姓名： | 刘永华 李国景 吴晓花 汪宝根 鲁忠富 朱祝军 |
| |
| 作者单位： | 1. 浙江省农业科学院,蔬菜研究所,浙江,杭州,310021
2. 浙江大学,农业与生物技术学院,浙江,杭州,310019 |
| |
| 基金项目： | 浙江省科技厅资助项目;浙江省科技厅资助项目 |
| |
| 摘 要： | 通过对影响长豇豆AFLP分析技术体系的若干关键因素,如DNA的提取方法、酶切与连接时间、预扩增和选择性扩增以及电泳和染色条件等的研究,建立一套经优化的长豇豆AFLP分析技术体系。应用该体系进行长豇豆AFLP分析,能够得到清晰的分子标记图谱,且结果稳定,重复性好。
|
| 关 键 词： | 长豇豆 分子标记 |
| 文章编号： | 1004-1524（2007）03-0156-04 |
| 收稿时间： | 2007-01-20 |
| 修稿时间： | 2007-01-20 |
Establishment and optimization of AFLP technological system for asparagus bean |
| |
| LIU Yong-hua,LI Guo-jing,WU Xiao-hua,WANG Bao-gen,LU Zhong-fu,ZHU Zhu-jun.Establishment and optimization of AFLP technological system for asparagus bean[J].Acta Agriculturae Zhejiangensis,2007,19(3):156-159. |
| |
| Authors: | LIU Yong-hua LI Guo-jing WU Xiao-hua WANG Bao-gen LU Zhong-fu ZHU Zhu-jun |
| |
| Institution: | 1 Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China ; 2 Collage of Agricultural and Biotechnology , Zhejiang University, Hangzhou 310019, China |
| |
| Abstract: | Several main factors affecting the results of AFLP fingerprints for asparagus bean(Vigna unguiculata ssp.sesquipedlis(L.)Verd.) were studied,which including the method of genomic DNA extraction,enzyme digestion and ligation, pre-amplification and selective-amplification,electrophoresis,dyeing conditions etc.An optimized AFLP analysis system was established.Using this system,a clear,credible and repeatable AFLP fingerprints could be obtained. |
| |
| Keywords: | AFLP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
