| 兔类胚胎干细胞的体外培养 |
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| 引用本文： | 陈晓宇,朱志伟,于福先,潘建治.兔类胚胎干细胞的体外培养[J].浙江农业学报,2011,23(2):0. |
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| 作者姓名： | 陈晓宇 朱志伟 于福先 潘建治 |
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| 作者单位： | 浙江省农业科学院 畜牧兽医研究所,浙江 杭州 310021 |
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| 基金项目： | 浙江省自然科学基金，浙江省科技厅面上项目，浙江省农业科学院科技创新提升工程 |
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| 摘 要： | 为了确立兔类胚胎干细胞(ESCs-like)体外培养方法,研究探讨了兔囊胚形成环境与饲养层细胞对内细胞团(ICM)增殖、ICM原代集落形成以及集落碱性磷酸酶(AKP)阳性率的影响,并在MEF和REF饲养层上进行了兔ESCs-like的体外培养。结果显示,去除透明带桑葚胚在体外具有较高的囊胚发育率和囊胚贴壁率(P<0.05),体外发育囊胚ICM原代集落的AKP阳性率却显著低于体内发育囊胚(P<0.05)；与MEF饲养层相比,REF饲养层上ICM原代集落的形成率显著偏低(P>0.05),但原代集落的AKP阳性率却显著高于其余各组(P<0.05)；MEF饲养层与REF饲养层均支持兔ESCs-like的传代培养,但F3代后MEF组中ESCs-like的集落形成率显著高于REF组(P<0.05)。
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| 关 键 词： | 类胚胎干细胞(ESCs-like) 内细胞团(ICM) 集落 饲养层 兔 |
Culture of rabbit ESCs-like in vitro |
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| CHEN Xiao-yu,ZHU Zhi-wei,YU Fu-xian,PAN Jian-zhi.Culture of rabbit ESCs-like in vitro[J].Acta Agriculturae Zhejiangensis,2011,23(2):0. |
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| Authors: | CHEN Xiao-yu ZHU Zhi-wei YU Fu-xian PAN Jian-zhi |
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| Institution: | Institute of Animal Husbandry and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
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| Abstract: | To develop a method of rabbit ESCs-like(embryonic stem cells like) culture in vitro for obtaining the rabbit ESCs(embryonic stem cells) line in future,not only the rates of inner cell mass(ICM) proliferation,formation and positive alkaline phosphatase(AKP) of the primary rabbit ICM colonies from in vitro and in vivo were comparativly studied on different feeders,but also rabbit ESCs-like were passage cultured separately on MEF feeder and REF feeder.The results showed that there were significantly higher rat... |
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| Keywords: | embryonic stem cells like(ESCs-like) inner cell mass(ICM) colonies feeder rabbit |
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