鲟海豚链球菌的分离鉴定及其感染后的病理损伤

2016年6月,四川彭州养殖鲟鱼流行一种以神经症状和出血为临床特征的传染病,组织病理学观察发现,发病鲟鱼全身多组织器官都有明显的病理损伤,尤其是在肝、肾、心和脑表现为明显的变性、坏死、出血以及炎症细胞浸润。为明确其病因,从自然发病鱼的内脏进行病原菌分离,根据分离菌的形态、理化特性,结合16S rRNA系统发育分析和特异性PCR检测,将其鉴定为海豚链球菌(Streptococcus iniae)。并在此基础上进行人工感染试验,证实其病原性。药敏结果显示,其对氟苯尼考、庆大霉素、强力霉素等抗菌药物高度敏感,但对四环素、新霉素不敏感。

Isolation and identification of Streptococcus iniae from sturgeon and its pathological lesions of infection

During June of 2016, a serious infectious disease characterized by hemorrhage and neurological symptoms outbroke in sturgeon farms of Pengzhou, Sichuan Province. The main pathologic lesions were degeneration, necrosis, hemorrhage and infiltration of the inflammation cells that was particularly marked in liver, kidney, heart and brain. A pathogenic bacteria was isolated from the viscera of sick sturgeons and the isolate was identified as Streptococcus iniae by physiological and biochemical characteristics, phylogenetic analysis and specific PCR detection, its pathogenicity was confirmed by artificial infection test. The drug sensitivity results showed that the isolate was sensitive to florfenicol, gentamicin, doxycycline, etc., but resistant to tetracycline and neomycin.