大熊猫源枝孢样枝孢霉野生株(Z20)与突变株(Zt)部分生物学特性及药敏试验比较

为探明枝孢样枝孢霉野生株Z20与突变株Zt在生物学特性及药物敏感上的差异,将枝孢样枝孢霉Z20和Zt于PDA培养基上连续培养7 d,每天观察菌落形态及长势。将菌株Z20和Zt在沙氏液基上培养4 d后用透射电镜对其超微结构进行观察。采用硫酸-苯酚法测定枝孢样枝孢霉Z20和Zt的菌丝体多糖含量。运用96孔板微量稀释法测定5种常见抗真菌药物对枝孢样枝孢霉Z20和Zt的最小抑菌浓度。结果表明:在PDA培养基上菌株Zt的生长速率高于Z20,菌落颜色较浅,孢子数量减少。在透射电镜下,野生株Z20的菌丝壁厚度和菌丝宽度显著大于突变株Zt,且菌丝的含糖量也显著高于突变株Zt。药敏试验中,除灰黄霉素外,枝孢样枝孢霉Zt对5种药物的敏感性均高于Z20。

Comparison of partial biological characteristics and drug sensitivity between Cladosporium cladosporioides wild strain (Z20) from giant panda and mutant strain (Zt)

In order to explore the difference of biological characteristics and drug sensitivity of Cladosporium cladosporioides (Z20) and the mutant strain (Zt), Z20 and Zt were continuously incubated on PDA medium for 7 d, and the colony morphology and growth information were observed and recorded every day. The ultrastructure of Z20 and Zt were observed under the transmission electron microscope. The polysaccharide contents of Z20 and Zt were detected by sulfuric acid-phenol method. The minimum inhibitory concentrations (MICs) of five commonly used antifungal agents against Cladosporium cladosporioides Z20 and Zt were determined by 96-well plate microdilution method. The results showed as follows: The growth rate of Zt was higher than Z20, the colour of colony was shallow, but the number of spores decreased on PDA by continuously incubate. Under the transmission electron microscope, the mycelial wall thickness and mycelial width of wild strain Z20 were significantly greater than those of mutant strain Zt, and sugar content of mycelium was also significantly higher than that of mutant strain Zt. Antifungal susceptibility test showed that except griseofulvin, the sensitivity of mutant strain Zt to five drugs were higher than that of the Z20.