| 浙江省水稻品种DNA指纹数据库的初步构建及其应用 |
| |
| 引用本文： | 程本义,吴伟,夏俊辉,刘鑫,庄杰,杨仕华.浙江省水稻品种DNA指纹数据库的初步构建及其应用[J].浙江农业学报,2009,21(6). |
| |
| 作者姓名： | 程本义 吴伟 夏俊辉 刘鑫 庄杰 杨仕华 |
| |
| 作者单位： | 1. 中国水稻研究所,浙江,杭州,310006
2. 浙江省种子总站,浙江,杭州,310020 |
| |
| 基金项目： | 浙江省"三农五方"科技协作项目，中央级公益性科研院所专项资金项目 |
| |
| 摘 要： | 利用前期研究建立的一套水稻品种DNA指纹检测技术体系,对2002-2006年浙江省主要的98个水稻品种、以及2007-2008年155个浙江省区试水稻品种(181个次,含26个续试品种)进行了DNA指纹检测,构建了279个次水稻品种×12个微卫星标记的DNA指纹图谱数据库.系统分析了指纹库中各标记的多态性和品种的相似性.基于构建的指纹数据库,对所有品种的特异性及区试续试品种年度间的一致性进行了鉴定.结果发现5组11个品种组内品种间特异性不明显,1个续试品种年度间鉴定结果不一致.
|
| 关 键 词： | 水稻品种 微卫星标记 指纹库 特异性 |
Construction and application of DNA fingerprint database of rice varieties in Zhejiang Province |
| |
| CHENG Ben-yi,WU Wei,XIA Jun-hui,LIU Xin,ZHUANG Jie-yun,YANG Shi-hua.Construction and application of DNA fingerprint database of rice varieties in Zhejiang Province[J].Acta Agriculturae Zhejiangensis,2009,21(6). |
| |
| Authors: | CHENG Ben-yi WU Wei XIA Jun-hui LIU Xin ZHUANG Jie-yun YANG Shi-hua |
| |
| Abstract: | A total of 98 major rice varieties in Zhejiang Province from 2002 to 2006 and 155 rice varieties in Zhejiang provincial regional trial from 2007 to 2008 were assayed with a set of DNA fingerprint testing system on rice variety. A DNA fingerprint database containing genotypic data of 279 rice variety times 12 marker loci was constructed. The polymorphism of different markers and genetic similarity of varieties in the DNA fingerprint database were analyzed. Based on the DNA fingerprint database,the distinctness of all rice varieties and consistency of the same varieties in different years were identified. It was found that Ⅱ varieties of 5 groups had no obvious distinctness within groups ,and discrepancy existed in the same variety in different years. |
| |
| Keywords: | DNA rice variety microsatellite marker DNA fingerprint database distinctness |
| 本文献已被 万方数据 等数据库收录！ |
|
