氨氮降解微生物的筛选和初步应用

为了创制高效的粪污氨氮降解菌剂,特从猪粪中分离高效的氨氮降解微生物。通过感官法初筛,并结合氨氮降解率为指标进行复筛,最终获得高效氨氮降解菌株——Z2、AOZ和Y。经形态学观察、生理生化鉴定、16S rRNA基因序列分析,确定菌株Z2为弯曲芽孢杆菌(Bacillus flexus),菌株AOZ为恶臭假单胞菌(Pseudomonas putida),Y为干酪乳杆菌(Lactobacillus casei)。试验结果显示,菌株Z2和Y组成的复配菌剂对氨氮的降解效果最优,氨氮降解率高达53.19%,表明该复合菌剂可以应用于粪污除氨。

Screening and utilization of ammonia-nitrogen-degrading microorganism

To obtain the ammonia-lowering agent for livestock manure, the bacterial strains with good ammonia-nitrogen-degrading capability were isolated from swine manure. Three strains with high ammonia-nitrogen degradation efficiency were identified with the olfactory threshold method combined with the comparisons of their ammonia-nitrogen degradation efficiencies, and were encoded as Z2, AOZ and Y, respectively. Based on the results of morphological observation, physiological and biochemical tests, and 16S rRNA sequence homology analysis, the strains Z2, AOZ and Y were identified as Bacillus flexus, Pseudomonas putida, and Lactobacillus casei, respectively. The experimental results showed that the ammonia-nitrogen degradation efficiency was higher when strains Z2 and Y were mixed, and the highest ammonia-nitrogen degradation efficiency reached 53.19%, suggesting that the microorganism complex could be practically applied to remove ammonium from swine manure.