烤后健康烟叶和霉烂烟叶真菌群落结构分析

为了解烤后健康烟叶和霉烂烟叶真菌群落多样性,采用Illumina MiSeq测序平台对烤后健康与霉烂烟叶样品上真菌群落的ITS1-ITS2区域PCR扩增片段进行测序并分析。健康烟叶组(MJ)和霉烂烟叶组(MB)2类样品真菌主要为子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、接合菌门(Zygomycota)等6个门25纲58目187科260个属的394个真菌分类单元(OTUs)。在属水平上,健康烟叶组(MJ)优势真菌为链格孢属(Alternaria),其次为曲霉属(Aspergillus)、新凸轮孢菌属(Neocamarosporium)、赤霉属(Gibberella)、支顶孢属(Acremonium);霉烂烟叶组(MB)优势真菌为曲霉属(Aspergillus),其次为青霉属(Penicillium)和根霉属(Rhizopus)。Alpha指数表明,健康烟叶组(MJ)真菌群落丰富度与多样性均高于霉烂烟叶组(MB)。71.06%的OTUs归属于8个功能类群,其中腐生菌是主要功能类群,研究结果揭示了烤后健康烟叶和霉烂烟叶真菌群落结构,为制定相应的防治方案提供参考依据。

Fungal composition and diversity analysis of healthy and rotten tobacco leaves after curing

To explore difference of the fungal community composition of healthy and rotten tobacco leaves after curing, the fungal community composition of both healthy and tobacco pole rot samples from flue-cured tobacco leaves were investigated by using PCR amplification of the ITS1-ITS2 region. The amplified fragments were sequenced using Illumina Miseq high-throughput sequencing technique. It was shown that the fungi from healthy tobacco (MJ), rotten tobacco (MB) were distributed in 6 phyla (Ascomycota, Basidiomycota, Zygomycota, etc), 25 classes, 58 orders, 187 families, 260 genera and 394 fungal taxa (OTUs). The dominant genus of MJ was Alternaria, followed by Aspergillus, Neocamarosporium, Gibberella and Acremonium. For MB, the dominant genus was Aspergillus, followed by Penicillium and Rhizopus. Alpha index showed that the fungal community richness and diversity of MJ were higher than those of MB. Additionally, functional assignments were made, the results showed that 71.06% of total OTUs belonged to eight trophic modes, of which saprotroph was the main group. The results of this study revealed the community structure of healthy and rotten tobacco leaves after curing and provided reference basis for formulating the corresponding control plan.