不同植物生长调节剂对柿砧亚林6号硬枝扦插生根的影响

从幼化的砧木采穗圃采集亚林6号插穗,进行硬枝扦插生根试验。利用植物生长调节IBA、ABT1、NAA、GGR6、IAA剂配制的不同浓度溶液及作为对照的清水浸泡插条切口,而后扦插于无菌基质。遮阴拱膜保湿环境下抚育45 d及露天滴灌环境下抚育15 d后,对生根效果进行取样检测、差异显著性分析和多重比较分析。结果表明,不同处理之间扦插生根效果差异显著,IAA、IBA、GGR6等在合适配比下有较高的生根率和萌梢生长量。其中处理A1、G3、G2生根率分别达到75.6%、56.9%和52.8%,均显著高于其他处理;生根率和愈伤发生率的规律不一致;生根类型有皮部生根、愈伤生根、混合生根3种类型;实验中扦插生根以皮部生根为主,混合生根为辅;IAA等处理有利于提高皮部生根发生,NAA、添加腐植酸的IBA等处理有利于促进愈伤组织生根。IAA 250 mg·L^-1溶液处理的扦插生根效果最好,GGR6 500 mg·L^-1扦插生根效果次之。综合成本优势和操作方便性,建议生产中使用GGR6 500 mg·L^-1处理培育柿砧木扦插苗。

Effects of different plant growth regulators on hardwood cuttage of persimmon rootstock Yalin 6

The cuttings of Yalin No.6 were collected from the nursery of immature rootstock, and the rooting experiment of hardwood cutting was carried out. IBA, ABT1, NAA, GGR6, IAA and other plant growth regulators were used to prepare different concentrations of solution and water was used as the control for the cutting immersion, then the cuttings were soaked and cut in sterile medium. After 45 days of shading film moisturizing environment and 15 days of open-air drip irrigation environment, the rooting effect was detected by sampling, significant difference analysis and multiple comparative analysis. The results showed that there were significant differences in rooting effect among different treatments. IAA, IBA and GGR6 had higher rooting rate and sprout growth under appropriate ratio. The rooting rates of A1, G3 and G2 were 75.6%, 56.9% and 52.8% respectively, which were significantly higher than those of other treatments. The changes of rooting rate and callus occurrence rate were not consistent. There are three types of rooting: cortex rooting, callus rooting and mixed rooting. In the experiment, the cutting rooting was mainly cortex rooting, supplemented by mixed rooting. IAA and other treatments can improve the cortex rooting, while NAA and IBA with humic acid could promote the rooting of callus. IAA and other treatments can improve the rooting rate of cortex, 250 mg · L^-1 IAA treatment had the best rooting effect, 500 mg · L^-1 GGR6 treatment was the second. Considering the cost advantage and operation convenience, it was suggested to use 500 mg · L^-1 GGR6 treatment to cultivate persimmon rootstock cuttings.