恩施产区湖北贝母乙酸乙酯组分挥发性成分与抗菌活性研究

为了研究恩施产区湖北贝母乙酸乙酯组分的抗菌活性和挥发性成分,以恩施产区湖北贝母乙酸乙酯组分为研究对象,运用滤纸片法测试对11株供试菌的抑菌活性;采用微量肉汤稀释法测定对供试菌的MIC值;运用GC-MS鉴定挥发性成分;运用气相色谱外标法测定贝母素乙含量。研究结果显示:湖北贝母乙酸乙酯组分对苏云金芽孢杆菌、溶藻弧菌、鲍曼不动杆菌有中度敏感抑制活性,其MIC值分别为2.37、5.93、11.86 mg·mL^-1;湖北贝母乙酸乙酯组分的抑菌活性与贝母素乙的抑菌活性存在显著性差异,推测贝母素乙不是湖北贝母乙酸乙酯组分中主要发挥抑菌活性的成分;从乙酸乙酯组分中共鉴定出16个挥发性代谢产物,主要成分为脂肪烃(4.55%)、脂肪酸(24.07%)、含氮生物碱(23.02%);湖北贝母乙酸乙酯组分中贝母素乙的含量为(16.19±0.16) mg·g^-1,加标回收率为96.58%,相对标准偏差为3.98%。湖北贝母乙酸乙酯组分的优良抑菌活性和抑菌广谱性,为开发优质植物来源的新型天然抗生素提供了重要参考。

Study on volatile components and antibacterial activity of ethyl acetate component of Fritillaria hupehensis Hsiao et K. C. Hsia

To study the volatile components and antibacterial activity of ethyl acetate component of F. hupehensis, the ethyl acetate component of F. hupehensis in Enshi region was selected as the research object. The filter paper method was used to test the antimicrobial activity against 11 clinically resistant bacteria. Microbroth dilution method was used to determine the MIC value of the tested bacteria. The volatile components were identified by GC-MS and the content of peiminine was determined by gas chromatography. The results showed that: the ethyl acetate had medium inhibitory activity against Bacillus thuringiensis, Vibrio alginolyticus XSBZ14, and Acinetobacter baumannii ATCC 19606, and it’s MIC values was 2.37, 5.93 and 11.86 mg·mL^-1 respectively; the antibacterial activitys of ethyl acetate fraction of Hubei Fritillaria was significantly different from that of peiminine, which was proved that peiminine was not the main antibacterial agent in the ethyl acetate component of Fritillaria hupehensis. A total of 16 volatile metabolites were identified, including aliphatic hydrocarbons (4.55%), fatty acids (24.07%) and alkaloids (23.02%). The content of peiminine in the ethyl acetate fraction of F. hupehensis was (16.19±0.06) mg·g^-1, and the average recovery was 96.58%, the relaitve standard deviation was 3.98%. Excellent and broad spectrum antibacterial activity of ethyl acetate component of F. hupehensis provided an important reference for the development of new natural antibiotics from plant sources.