多孢木霉HZ-31菌株侵染对野燕麦生理机制的影响

选用青海农田常见杂草野燕麦作为靶标杂草,盆栽接种后测定了多孢木霉HZ-31菌株对野燕麦体内各种生理指标的影响,分析了寄主与病原菌相互作用的生理变化机制。结果表明,多孢木霉HZ-31菌株侵染野燕麦后,对植株生理机制防御酶的影响是多方面的,PAL和POD活性增加,以抵御菌株侵染,其余生理防御指标包括MDA含量、CAT、PPO、SOD活性、可溶性蛋白含量和叶绿素含量则受到菌株侵染的破坏,含量减少、活性降低。被抑制的防御酶作用效应大于被促进的防御酶。HZ-31菌株的侵染钝化或抑制了野燕麦体内主要酶类,中断相应的酶促反应,引起植物广泛的代谢变化,包括细胞膜透性改变、干扰光合作用、蛋白质合成、酚类物质代谢等生理方面的变化。

Effect of Trichoderma polysporum HZ-31 infection on physiological mechanism of Avena fatua L.

Avena fatua L. as a common weed in the farmland of Qinghai Province, was selected as the target receptor. The effects of Trichoderma polysporum HZ-31 on various physiological indexes of wild oats were determined after potted inoculation, and the physiological mechanism of the interaction between host and pathogen was analyzed. The results showed that there were various effects of Trichoderma polysporum HZ-31 on the defense enzymes of physiological mechanism of Avena fatua L., PAL and POD activity increased to resist the infection of the strain. The other physiological defense indexes including MDA content, CAT activity, PPO activity, SOD activity, soluble protein content and chlorophyll content were destroyed by the infection of the strain. The effect of the inhibited defense enzyme was greater than that of the promoted defense enzyme. The infection of HZ-31 inactivated or inhibited the main enzymes in Avena fatua L., interrupted the corresponding enzymatic reaction, and caused a wide range of metabolic changes in plants, including changes in cell membrane permeability, interference in photosynthesis, protein synthesis, phenol metabolism and other physiological changes.