基于ISSR的野生桑树桑黄菌株遗传多样性分析

为保护开发野生桑树桑黄种质资源,对17个不同来源的野生桑树桑黄菌株开展种内遗传多样性分析,利用简单重复序列间扩增(ISSR)分子标记技术对桑黄菌株DNA进行扩增,分析扩增条带,利用NTSYS软件构建亲缘关系UPGMA聚类图。结果表明:16条ISSR引物中,有10条ISSR引物多态性丰富,条带清晰;10条引物共检测到904个位点,其中,多态性位点717个,多态性百分比79.3%。在DNA指纹图谱中,引物P5、P812扩增条带多态性最高。NTSYS-PC2.10e软件分析表明,17个桑树桑黄遗传相似系数为0.57~0.99。UPGMA聚类分析结果显示,在遗传相似系数(GS)约0.65处,可将17个桑树桑黄划分为2大类群: S4,S23,S26为一大类群,其余为一大类群。综上可知,桑树桑黄种质资源具有丰富的遗传多样性,ISSR分子标记可有效区分不同桑树桑黄菌株。

Genetic diversity of wild Sanghuangporus sanghuang based on ISSR molecular markers

In order to provide basis for protection and development of germplasm resources of wild Sanghuangporus sanghuang, the genetic diversity of 17 wild Sanghuangporus sanghuang strains was investigated in this study. The amplified products of 17 wild Sanghuangporus sanghuang strains DNA based on ISSR makers technique were used to analyze the genetic diversity of Sanghuangporus sanghuang. Cluster dendrogram of different samples were established based on the unweighted pair-group method with arithmetic mean (UPGMA) by NTSYS-pc software. The results showed that bands amplified by 10 ISSR primers among the 16 ISSR primers were clear and polymorphisms rich. 10 ISSR primers generated 904 loci, of which 717 loci were polymorphic. The percentage of polymorphisms was 79.3%. Bands amplified by ISSR-primers P5 and P812 had the highest polymorphisms in DNA fingerprint. The genetic similarity coefficients of 17 Sanghuangporus sanghuang strains ranged from 0.57 to 0.99. UPGMA cluster analysis showed that 17 Sanghuangporus sanghuang strains were divided into 2 groups at the level of genetic similarity coefficient (GS) about 0.65 in the cluster dendrogram. S4, S23, S26 were grouped together and the other 14 strains were in one group. Results of ISSR analysis revealed that germplasm resources of Sanghuangporus sanghuang had plentiful genetic diversity. ISSR method was efficient for distinguishing different strains of Sanghuangporus sanghuang.