京海黄鸡柔嫩艾美尔球虫感染对脾脏和盲肠IL-6、IL-8、CCLi2基因表达量的影响及其相关性

白介素6(IL-6)、白介素8(IL-8)和CC趋化因子配体2(CCLi2)3种细胞因子在炎症反应以及机体免疫方面发挥重要作用。为了揭示球虫感染对脾脏和盲肠中IL-6、IL-8和CCLi2的表达量的影响,以京海黄鸡(Gallus gallus)为试验材料,设球虫感染组和非感染的对照组,利用实时荧光定量PCR(qRT-PCR)检测了脾脏与盲肠组织中IL-6、IL-8和CCLi2的mRNA的相对表达量,比较同一组织感染组与对照组间3个基因表达量的差异,同时分析同一处理组的同一组织内、不同组织间3个基因表达量的相关性。研究结果表明:感染组脾脏组织IL-6、IL-8和CCLi2三个基因的表达量均显著高于对照组(P<0.05),其中IL-6基因达极显著水平(P<0.01);感染组盲肠组织中IL-6、IL-8和CCLi2的表达量均极显著地高于对照组(P<0.01)。相关性分析结果表明,感染组脾脏组织中IL-6、IL-8和CCLi2的表达量之间均存在极显著的正相关(P<0.01);在感染组盲肠组织中IL-8、CCLi2与IL-6基因的表达量存在极显著的正相关(P<0.01),但IL-8与CCLi2基因表达量相关不显著(P>0.05);在对照组脾脏组织中,IL-6、IL-8与CCLi2间存在极显著的正相关(P<0.01),其他基因间相关均不显著(P>0.05);对照组盲肠组织中IL-6、IL-8和CCLi2之间均存在极显著的正相关(P<0.01);此外,无论感染组还是对照组,脾脏和盲肠组织间3个基因的表达量相关性均不显著(P>0.05)。以上结果表明,IL-6、IL-8和CCLi2可能在京海黄鸡球虫感染过程中发挥重要作用,研究结果对进一步探讨这3个基因在球虫病抗病育种中的作用提供了依据,对京海黄鸡球虫病抗病育种具有重要意义。

Effect and correlation analysis of Eimeria tenella infection on IL-6, IL-8 and CCLi2 genes expression in spleen and caecum of Jinghai Yellow Chicken (Gallus gallus)

Interleukin 6 (IL-6), interleukin 8 (IL-8) and CC motif chemokine ligand 2 (CCLi2) play an important role in the inflammatory response and immune. In this study, in order to reveal the expression relationships between the three genes in spleen, caecum and between spleen and caecum at coccidiosis infected and non-infected state, Jinghai Yellow Chicken(Gallus gallus) were divided into coccidiosis infection and non-infection group, the relative expression of mRNA of IL-6, IL-8 and CCLi2 in spleen and caecum was detected by quantitative real-time polymerase chain reaction (qRT-PCR), the relative expression difference of the three genes were compared between infection and control group in the same tissue, meanwhile, the relative expression correlation were analyzed among the three genes in the same and different tissues within the same group. The results showed that the relative expression levels of IL-6, IL-8 and CCLi2 genes in spleen tissue of infected group were all higher than those of the control group (P<0.05), and the relative expression level of IL-6 gene in infected group was extremely significant different from control group (P<0.01); the relative expression levels of IL-6, IL-8 and CCLi2 genes in the caecum tissue of infected group were all extremely significant different from that of the control group (P<0.01). The correlation analysis showed that the correlation coefficient between the expression of IL-6, IL-8 and CCLi2 genes in spleen tissues of infected group were all positively related (P<0.01); in caecum tissue of infected group, there were extremely significant positive correlations(P<0.01) among the relative expression of the IL-8, CCLi2 with IL-6 genes, but the relative expression correlation between IL-8 and CCLi2 genes was not significant correlated (P>0.05); in the spleen tissue of the control group, there were extremely significant positive correlations (P<0.01) between the relative expression of IL-6, IL-8 with CCLi2 genes, and the correlation between other genes in the control group was not significant (P>0.05); there were extremely significant positive correlations (P<0.01) among the three genes of IL-6, IL-8 and CCLi2 in the caecum tissue of the control group; the correlation analysis results also showed that the expression of the three genes was not significantly correlated (P>0.05) between spleen and caecum tissue, regardless of the infection group or the control group. All of the results indicated that IL-6, IL-8 and CCLi2 might play an important role in the process of Jinghai Yellow Chicken coccidiosis infection, and the results provided the basis for further exploring the role of these three genes in the genetic breeding of coccidiosis resistance, and it had an important significance for Jinghai Yellow Chicken genetic breeding of coccidiosis resistance.