| 小麦TaeEF1β基因的克隆、同源性及表达分析 |
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| 引用本文： | 陈炫,张天烨,羊健,张恒木,陈剑平.小麦TaeEF1β基因的克隆、同源性及表达分析[J].浙江农业学报,2019,31(1):98. |
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| 作者姓名： | 陈炫 张天烨 羊健 张恒木 陈剑平 |
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| 作者单位： | 1.浙江农林大学 林业与生物技术学院,浙江 杭州 311300; 2.浙江省农业科学院 病毒学与生物技术研究所,浙江 杭州 310021 |
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| 基金项目： | 国家自然科学基金(3150160); 国家农业产业体系(CARS-3-1); 国家小麦转基因专项(2016ZX08002001) |
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| 摘 要： | 真核翻译延伸因子(eukaryotic translation elongation factor,eEFs)是一种重要的多功能调控蛋白,eEF1β是eEF1的组成部分,在蛋白质生物合成过程中发挥着重要的作用。本文通过RT-PCR扩增克隆小麦(Triticum aestivum L.)的eEF1β基因,并命名为TaeEF1β。氨基酸同源性分析发现,TaeEF1β具有高度保守性,且其保守结构域位于137~226 aa处。qRT-PCR结果表明,中国小麦花叶病毒(Chinese wheat mosaic virus,CWMV)侵染小麦植株后,可以诱导TaeEF1β基因转录水平的上调表达。另外,本文也进一步分析了TaeEF1β基因在小麦根、茎、叶的表达水平和CWMV侵染不同时间点的表达情况。
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| 关 键 词： | 真核翻译延伸因子 中国小麦花叶病毒 同源性分析 |
| 收稿时间： | 2018-03-25 |
Gene cloning,homology and expression analysis of TaeEF1β in Triticum aestivum L. |
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| CHEN Xuan,ZHANG Tianye,YANG Jian,ZHANG Hengmu,CHEN Jianping.Gene cloning,homology and expression analysis of TaeEF1β in Triticum aestivum L.[J].Acta Agriculturae Zhejiangensis,2019,31(1):98. |
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| Authors: | CHEN Xuan ZHANG Tianye YANG Jian ZHANG Hengmu CHEN Jianping |
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| Institution: | 1.College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China;
2.Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
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| Abstract: | Eukaryotic translation elongation factor (eEFs) is an important multifunctional protein. eEF1β was subunit of the eukaryotic translation elongation factor-1 (eEFs-1) complex and played an important role in protein biosynthesis. The eEF1β gene was obtained by cloning with RT-PCR from wheat and named as TaeEF1β. The amino acid sequences of TaeEF1β were highly conserved and the conserved domain was located in 137-226 aa. The results of qRT-PCR showed that TaeEF1β were up-regulated in the CWMV-infected plants. In this study, the expression of TaeEF1β in the stems, leaves and roots of wheat and different stages of CWMV infection was also detected by qRT-PCR. |
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| Keywords: | eukaryotic translation elongation factor Chinese wheat mosaic virus homology analysis |
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