金针菇生长发育期间相关胞外酶的活性变化研究

以白色金针菇菌株为材料,研究其生长发育过程中羧甲基纤维素酶、半纤维素酶、漆酶、酸性磷酸酶和中性蛋白酶活性的变化规律.结果表明,整个生长发育期间胞外酶活性变化具有明显的阶段性.羧甲基纤维素酶菌丝营养生长阶段活性较低,现蕾后活性急剧增强,达到11.43U；半纤维素酶高峰出现于采收期,达到8.13U；漆酶在菌丝营养生长阶段活性较高,原基形成后迅速下降到0.15U；中性蛋白酶菌丝营养生长阶段活性比较稳定,现蕾前相对较低,子实体生长阶段酶活性明显增强；酸性磷酸酶活性随着菌丝培养呈下降趋势,但生殖生长期迅速升高.

Changes of several extracellular enzyme activities of Flammulina velutipes during its growth and development

Changes of five extracellular enzymes activity(carboxymethyl cellulose,hemicellulase,laccase,neutral proteinase and acid phosphatase) of Flammulina velutipes were investigated during its growth and development.The results showed that the activity curves of extracellular enzyme during the growth and development showed markedly periodic style.Carboxymethyl cellulose activity was relatively low during the mycelial vegetative growth phase but it reached 11.43 U after primordium formation.Hemicellulase activity was the highest of 8.13 U during the harvest phase.Laccase activity was relatively high during mycelial growth phase and decreased to 0.15 U after primordium formation.Neutral proteinase activity remained constant during mycelial vegetative growth phase,decreased to relatively low levels before primordium formation,and increased again during the fruit body growth phase.Acid phosphatase activity decreased with mycelium culture,but increased sharply after development formation.