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褐飞虱共生菌抗吡虫啉菌株和敏感菌株解毒酶活性的比较
引用本文:李娜,陈建明,张珏锋,何月平,陈列忠.褐飞虱共生菌抗吡虫啉菌株和敏感菌株解毒酶活性的比较[J].浙江农业学报,2010,22(5):653.
作者姓名:李娜  陈建明  张珏锋  何月平  陈列忠
作者单位:1.杭州师范大学 生命与环境科学学院,浙江 杭州310036;2.浙江省农业科学院 植物保护与微生物研究所,浙江 杭州310021
基金项目:国家自然科学基金,浙江省自然科学基金
摘    要:比较测定了褐飞虱共生菌抗吡虫啉菌株和敏感菌株3种解毒酶活性的变化。结果表明,褐飞虱共生菌不同抗感吡虫啉菌株3种解毒酶活性差异显著(P<0.05),其中抗吡虫啉菌株的羧酸酯酶、多功能氧化酶活性显著高于敏感菌株,而谷胱甘肽-S-转移酶活性差异不显著。说明褐飞虱共生菌对吡虫啉抗药性的提高,与其体内羧酸酯酶、多功能氧化酶的活性增强有关。

关 键 词:褐飞虱共生菌  抗吡虫啉菌株  敏感菌株  解毒酶活性  抗药性  

Comparison for activities of detoxifying enzymes in resistant- and susceptible-imidacloprid endosymbiotic strains of rice brown planthopper, Nilaparvata lugens St(a)l
LI Na,CHEN Jian-ming,ZHANG Jue-feng,HE Yue-ping,CHEN Lie-zhong.Comparison for activities of detoxifying enzymes in resistant- and susceptible-imidacloprid endosymbiotic strains of rice brown planthopper, Nilaparvata lugens St(a)l[J].Acta Agriculturae Zhejiangensis,2010,22(5):653.
Authors:LI Na  CHEN Jian-ming  ZHANG Jue-feng  HE Yue-ping  CHEN Lie-zhong
Institution:1 College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036, China; 2Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Abstract:Activities of detoxifying enzymes in resistant- and susceptible-imidacloprid endusymbiotic strains of rice brown planthopper, Nilaparvata lugens were compared. The results showed that resistant- and susceptible-imidacloprid strains had significant difference in activities of detoxifying enzymes, in which, activities of carboxylesterase, mixed function oxidase in resistant-imidacloprid strains were significantly higher than those in susceptible-imidacloprid strains, while activity of glutathione-S-transferase showed no obvious difference between resistant-and susceptible-imidacloprid strains. It was suggested that resistant increasing of N. lugens symbiotes to imidacloprid was related to enhencement of carboxylesterase, mixed function oxidase activities.
Keywords:Nilaparvata lugens endosymbiotes  resistant-imidacloprid strain  susceptible-imidacloprid strain  activity of detoxifying enzymes  resistance  
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