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新城疫GM2分离株蚀斑克隆纯化及毒力测定
引用本文:王友令,杨金兴,袁小远,徐怀英,秦卓明.新城疫GM2分离株蚀斑克隆纯化及毒力测定[J].浙江农业学报,2009,21(6).
作者姓名:王友令  杨金兴  袁小远  徐怀英  秦卓明
作者单位:山东省农业科学院,家禽研究所,山东,济南,250100
基金项目:山东省科技攻关项目,山东省农业科学院创新基金项目 
摘    要:新城疫(newcastle disease,ND)是世界公认的最重要的家禽疫病之一,伴随着NDV活疫苗的广泛使用,野外分离的新城疫病毒往往由多种强弱病毒混合而成.为了避免不同分离株干扰,必须通过蚀斑纯化将混杂在一起的不同NDV毒株区分开来.文章采用蚀斑纯化技术对野外分离毒新城疫GM2进行蚀斑纯化,并对蚀斑纯化前后病毒的生物学特性进行测定,结果显示,该分离毒蚀斑纯化前MDT,ICPI,IVPI分别为50.5,1.78,2.41,蚀斑纯化后MDT,ICPI,IVPI分别为47.5,1.89,2.65.

关 键 词:新城疫病毒  蚀斑纯化

Plaque-purification of newcastle disease GM2 isolates and determination of biological characteristics
WANG You-ling,YANG Jin-xing,YUAN Xiao-yuan,XU Huai-ying,QIN Zhuo-ming.Plaque-purification of newcastle disease GM2 isolates and determination of biological characteristics[J].Acta Agriculturae Zhejiangensis,2009,21(6).
Authors:WANG You-ling  YANG Jin-xing  YUAN Xiao-yuan  XU Huai-ying  QIN Zhuo-ming
Abstract:Newcastle disease (ND) is recognized as one of the most important poultry diseases in the world. Along with the extensive use of live vaccine against NDV, newcastle disease virus isolated from the field is often a combination of different newcastle disease. In order to avoid the interference among different strains,it is necessary to purify and distinguish the different NDV strains through the plaque. Newcastle GM2 isolates were purified by plaquepurification in this study,and the biological characteristics of the virus before and after plaque-purification were determined. The result showed that MDT, ICPI,IVPI of the Newcastle GM2 isolates before plaque purification was 50.5, 1.78 and 2.41 ,whereas after plaque-purification MDT, ICPI, IV PI was 47.5,1.89 and 2.65, respectively.
Keywords:newcastle disease virus  plaque-purification
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