| 甜瓜枯萎病菌ELISA检测方法的初步建立 |
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| 引用本文： | 延涵,杨瑞秀,盖晓彤,李刚,赵治国,高增贵.甜瓜枯萎病菌ELISA检测方法的初步建立[J].浙江农业学报,2018,30(12):2081. |
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| 作者姓名： | 延涵 杨瑞秀 盖晓彤 李刚 赵治国 高增贵 |
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| 作者单位： | 1.内蒙古出入境检验检疫局 检验检疫技术中心,内蒙古 呼和浩特 010020;2.沈阳农业大学 植物免疫所,辽宁 沈阳 110866 |
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| 基金项目： | 公益性行业(农业)科研专项(201503110-04) |
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| 摘 要： | 采用甜瓜枯萎病病菌菌丝蛋白作为抗原,制备该病菌菌丝蛋白的多克隆抗体,建立检测甜瓜枯萎病菌的间接ELISA检测方法。利用检测技术对田间病样和土壤中的病原菌含量进行了定性、定量检测,表现出方便、特异性强、灵敏度高的特点。可运用此方法对甜瓜枯萎病进行田间检测。
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| 关 键 词： | 甜瓜枯萎病菌 多克隆抗体 ELISA |
| 收稿时间： | 2018-03-28 |
Establishment of ELISA for Fusarium oxysporum f. sp. melonis |
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| YAN Han,YANG Ruixiu,GAI Xiaotong,LI Gang,ZHAO Zhiguo,GAO Zenggui.Establishment of ELISA for Fusarium oxysporum f. sp. melonis[J].Acta Agriculturae Zhejiangensis,2018,30(12):2081. |
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| Authors: | YAN Han YANG Ruixiu GAI Xiaotong LI Gang ZHAO Zhiguo GAO Zenggui |
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| Institution: | 1. Inspection and Quarantine Technology Center, Inner Mongolia Entry-Exit Inspection Bureau, Hohhot 010020, China;
2. Institute of Plant Immunity, Shenyang Agricultural University, Shenyang 110866, China |
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| Abstract: | Polyclonal antibody was prepared by the mycelium protein of Fusarium oxysporum f. sp. melonis as antigen, and indirect ELISA method was established. The field samples and soil samples were detected by qualitative and quantity analysis, which showed charasteristics of convenience, specificity and high sensitivity. The ELISA detection technology could be easily applied in the field and have important practical value. |
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| Keywords: | Fusarium oxysporum f sp melonis polyclonal antibody ELISA |
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