二次正交旋转组合设计优化热水法提取香菇废菌棒多糖工艺的研究

采用四因素二次回归正交旋转组合设计方法，研究浸提温度、浸提时间、液固比和醇沉比对香菇废菌棒多糖的影响，确定香菇废菌棒多糖的较优提取工艺。结果表明，所得回归方程达到极显著水平，无失拟因素存在。通过频率分析法得到香菇废菌棒多糖的最优热水提取工艺为浸提温度92℃，浸提时间6 h 18 min，液固比40∶1，醇沉比例3∶1，在此条件下香菇废菌棒多糖提取率为1868 mg·g-1。

Optimization of hot\|water extraction conditions for polysaccharide in Lentinus edodes waste substrate by quadratic regression rotation\|orthogonal combination design

Four factors quadratic orthogonal rotation design was used to investigate the influencing factors on the yields of polysaccharide from Lentinus edodes waste substrate such as extraction temperature， extraction time， liquid/solid ratio and alcohol precipitation ratio. The results indicated that the obtained regression equation reached the significant level without any lack of fit factor. The optimum extraction conditions were as follows: temperature 92℃ for six hours and 18 minutes， liquid/solid ratio 40∶1， and alcohol precipitation ratio of 3∶1. The yield of the polysaccharides of Lentinus edodes waste substrate was 1868 mg·g-1 under above conditions.