| 芥蓝Brassica albograbra花青素合成酶基因BaANS的克隆与序列分析 |
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| 引用本文： | 赵蓉蓉,蒋明,贺蔡明,朱雅琴,周敏.芥蓝Brassica albograbra花青素合成酶基因BaANS的克隆与序列分析[J].浙江农业学报,2010,22(2). |
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| 作者姓名： | 赵蓉蓉 蒋明 贺蔡明 朱雅琴 周敏 |
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| 作者单位： | 台州学院,生命科学学院,浙江,临海,317000 |
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| 基金项目： | 浙江省自然科学基金，台州市科技计划项目 |
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| 摘 要： | 根据NCBI数据库中的已知序列设计简并引物,分别从芥蓝(Brassica albograbra)叶片基因组DNA和cDNA中克隆到了花青素合成酶基因。基因命名为BaANS,该基因全长1369bp,具一个长度为292bp的内含子,编码区长度为1077bp,编码358个氨基酸,序列已提交到NCBI,登录号为GU170203。序列比对结果表明,BaANS与甘蓝、拟南芥、紫罗兰、白菜和芥菜等的ANS有较高的相似性。
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| 关 键 词： | 芥蓝 花青素合成酶 序列分析 |
Isolation and sequence analysis of anthocyanidin synthase gene BaANS from Brassica albograbra |
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| ZHAO Rong-rong,JIANG Ming,HE Cai-ming,ZHU Ya-qin,ZHOU Min.Isolation and sequence analysis of anthocyanidin synthase gene BaANS from Brassica albograbra[J].Acta Agriculturae Zhejiangensis,2010,22(2). |
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| Authors: | ZHAO Rong-rong JIANG Ming HE Cai-ming ZHU Ya-qin ZHOU Min |
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| Institution: | ZHAO Rong-rong,JIANG Ming*,HE Cai-ming,ZHU Ya-qin,ZHOU Min (College of Life Science,Taizhou University,Linhai 317000,China) |
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| Abstract: | Degenerate primer pairs were designed according to known anthocyanidin synthase gene sequences in NCBI database.The gene,designated BaANS,was isolated from leaf genomic DNA and cDNA of Chinese kale (Brassica albograbra),respectively.Genomic DNA of BaANS gene is 1 369 bp in length with one intro of 292 bp.Complete coding sequence of BaANS is 1 077 bp in length encoding 358 amino acids.The sequence has been submitted to NCBI with accession number of GU170203.Sequence comparison with Matthiola incana,B.olerace... |
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| Keywords: | Chinese kale (Brassica albograbra) anthocyanidin synthase sequence analysis |
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