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三种海藻抑菌活性和抗氧化活性
引用本文:钱卓权,殷皓臻,黄晓林,林少珍,南海函. 三种海藻抑菌活性和抗氧化活性[J]. 浙江农业学报, 2014, 26(2): 0
作者姓名:钱卓权  殷皓臻  黄晓林  林少珍  南海函
作者单位:1.温州医学院 生命科学学院,温州 浙江 325035;2浙江省海洋水产养殖研究所,温州 浙江 325000
基金项目:浙江省近岸水域生物资源开发与保护重点实验室资助(J2011006);浙江省海洋与渔业计划项目(浙海渔计[2011]123号);浙江省科技厅科技计划项目(2011F20033)
摘    要:以药敏纸片扩散法检测3种大型海藻鼠尾藻(Sargassum thunbergii)、羊栖菜(Sargassum fusiforme)和海带(Laminaria japonica)提取物对大肠杆菌、金黄色葡萄球菌和铜绿假单胞菌的抑制作用,以DPPH清除自由基方法检测3种海藻提取物的抗氧化能力。结果表明,3种海藻提取物对金黄色葡萄球菌均有较显著抑制作用,且以鼠尾藻正丁醇提取物的抑菌效果最佳;鼠尾藻提取物抗氧化能力较强,其中乙酸乙酯提取物的抗氧化活性比石油醚和正丁醇提取物强。

关 键 词:海藻  抑菌活性  DPPH  抗氧化能力  

Antibacterial and antioxidant bioassay of three kinds of algae
QIAN Zhuo quan,YIN Hao zhen,HUANG Xiao lin,LIN Shao zhen,NAN Hai han. Antibacterial and antioxidant bioassay of three kinds of algae[J]. Acta Agriculturae Zhejiangensis, 2014, 26(2): 0
Authors:QIAN Zhuo quan  YIN Hao zhen  HUANG Xiao lin  LIN Shao zhen  NAN Hai han
Affiliation:1 School of Life Sciences, Wenzhou Medical College, Wenzhou 325035, China; 2Zhejiang Mariculture Research Institute, Wenzhou 325000, China
Abstract:The antibacterial activity of extracts of Sargassum thunbergii, Sargassum fusiforme and Laminaria japonica against Colon bacillus, Staphylococcus aureus and Pseudomonas aeruginosa was tested by drug sensitive disk diffusion method. Antioxidant activities of these extracts were analyzed by DPPH free radical scavenging test. The extracts of three kinds of algae showed significant antibacterial activities against S. aureus. N butanol extracted from S. thunbergii had the highest antibacterial effects. DPPH tests revealed the extracts of S. thunbergii were more significantly antioxidant than extracts of other algae. And the antioxidant activity of the ethyl acetate extract of S. thunbergii was stronger than those of petroleum ether and n butanol extracts of S. thunbergii.
Keywords:algae  antibacterial activity  DPPH  antioxidant bioassay  
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