|
|||||
|
|
| 利用胶体金免疫层析法检测猪瘟强、弱毒抗体方法的建立 | |
| 引用本文： | 郑鸣,金彦法,闫国庆.利用胶体金免疫层析法检测猪瘟强、弱毒抗体方法的建立[J].郑州牧业工程高等专科学校学报,2006,26(2):1-3,13. |
| 作者姓名： | 郑鸣 金彦法 闫国庆 |
| 作者单位： | 1. 郑州牧业工程高等专科学校,生物工程系,河南,郑州,450011 2. 河南海润实业总公司猪场,河南,焦作,454100 3. 东北农业大学,黑龙江,哈尔滨,150001 |
| 基金项目： | 河南省自然科学基金(O211031300) |
| 摘 要： | 将猪瘟病毒(Hog Cholera Virus,HCV)E2蛋白用大肠杆菌表达纯化后用作弱毒抗原；猪瘟病毒(HCV)强毒株用PK-15细胞培养48h,破碎后进行蔗糖密度梯度离心,吸取30％～35％梯度之间的条带,纯化后用作强毒抗原,然后用胶体金标记抗原,运用双抗原夹心法于国内首先建立了HCV抗体检测的免疫层析试纸条。该方法操作简单、灵敏度高、特异性好,能区分强弱毒感染,适合猪瘟的临床诊断。 |
| 关 键 词： | 猪瘟病毒 基因表达 细胞培养 胶体金 |
| 文章编号： | 1008-3111（2006）02-0001-03 |
| 收稿时间： | 2006-01-07 |
| 修稿时间： | 2006-01-07 |
Establishment of colloidal gold immune chromatographic assay for detection of hog choleras attenuated and virulent strain virus antibody |
|
| ZHENG Ming,JIN Yan-fa,YAN Guo-ping.Establishment of colloidal gold immune chromatographic assay for detection of hog choleras attenuated and virulent strain virus antibody[J].Journal of Zhengzhou College of Animal Husbandry Engineering,2006,26(2):1-3,13. | |
| Authors: | ZHENG Ming JIN Yan-fa YAN Guo-ping |
| Institution: | 1. Department of Bioengineering of Zhen~zhou College of Animal Husbandry and Engineering, Zhengzhou Henan 450011 ,China;2. Pig farm of Head office of the industry of Henan hai run,Jiaozuo Henan 454100,China ;3. Northeast Agricultural University, Haerbin Hendongjiang, 150001,China |
| Abstract: | Protein E2 of Hog Cholera Virus was expressed in E. coll. After purified, the gene- expressed E2 acts as attenuated HCV antigen. The PK- 15 cells infected by HCV titration of virulent strain were cultivated for 48 hours. Then the PK- 15 cells were braked and performed a sucrose density gradient centrifugation. The proteins between 30% -- 35% gradient were purified and served as virulent HCV antigen. The HCV antigens were labeled by colloidal gold. Based - on sandwich ELISA technique,immune chromatographic assay test paper for detecting HCV is firstly developed in China. The method can discriminate between virulent and attenuated strain, which is more sensitive and specific than other methods and fit for clinical diagnosis of HCV. |
| Keywords: | HCV gene expression cell culture colloidal gold |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |