青枯雷尔氏菌致病性生物测定方法的研究

采用不同浓度的强致病力青枯雷尔氏菌FJAT-91处理番茄盆栽苗,建立青枯雷尔氏菌致病性生物测定方法.结合菌落形态和特异性引物鉴定结果说明所分离得到的菌株均为强致病力青枯雷尔氏菌.当番茄植株培养至第5d时,4个不同接种浓度处理的番茄植株苗均出现发病症状.采用104 cfu·mL-1处理的番茄植株在第4d发病,但到第8d,...

Study on the Bioassay Method for Pathogenicity of Ralstonia solanacearum

The bioassay method of virulent Ralstonia solanacearum strain was established on the tomato plants treated by different concentration of virulent R.solanacearum strain FJAT-91.The results showed that the R.solanacearum strains isolated from wilted tomato plants were virulent strains according to the colony form and test of polymerase chain reaction(PCR) with special primers.The disease symptom of tomato plants treated with different concentrations appeared on the fifth day.The tomato plants began wilting on the fourth day,which were treated with concentration of 104 cfu·mL-1,and the incidence was reached 90% after 8 days.Time to LC50 of tomato plants was shortened along with the increasing of concentration of R.solanacearum.Therefore,the concentration of 104 cfu·mL-1 is the optimal one that could shorten test period and make the tomato plant wilt.