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福建省大豆根腐病病原菌的鉴定
引用本文:李本金,陈庆河,兰成忠,王娜娜,王源超,翁启勇.福建省大豆根腐病病原菌的鉴定[J].福建农业学报,2011,26(5):798-803.
作者姓名:李本金  陈庆河  兰成忠  王娜娜  王源超  翁启勇
作者单位:1. 福建省农业科学院植物保护研究所,福建福州,350013
2. 南京农业大学植物病理学系,江苏南京,210095
基金项目:国家大豆产业技术体系项目,福建省农科教项目,福建省自然科学基金,福建省财政专项——福建省农业科学院科技创新团队建设基金
摘    要:为明确福建省大豆根腐病病原菌的种类及组成,于2007~2009年从漳州、厦门、龙岩等地大豆产区采集根腐病标样216份,从中分离到307株丝状病原真菌.根据其培养性状、形态学特征、代表性菌株的致病性测定及rDNA-ITS序列分析,将这些病原菌分为5种,分别为尖孢镰刀菌(Fusarium oxysporum,112株)、茄...

关 键 词:大豆根腐病  病原鉴定  ITS  致病性

A Identification and Pathogenicity Test of the Pathogens Causing Soybean Root Rot in Fujian
LI Ben-jin,CHEN Qing-he,LAN Cheng-zhong,WANG Na-na,WANG Yuan-chao,WENG Qi-yong.A Identification and Pathogenicity Test of the Pathogens Causing Soybean Root Rot in Fujian[J].Fujian Journal of Agricultural Sciences,2011,26(5):798-803.
Authors:LI Ben-jin  CHEN Qing-he  LAN Cheng-zhong  WANG Na-na  WANG Yuan-chao  WENG Qi-yong
Institution:1(1.Institute of Plant Protection,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China; 2.Department of Plant Pathology,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China)
Abstract:Two hundred and sixteen soybean root rot samples were collected from Zhangzhou,Xiamen,Longyan city in Fujian province during 2007 to 2009 in order to determine the fungal pathogens that could cause soybean root rot,and three hundred and seven filamentous fungi strains were isolated from the samples.Five groups of pathogen were identified,which were Fusarium oxysporum(112 isolates),Fusarium solani(74 isolates),Phytophthora sojae(79 isolates),Rhizoctonia solani(24 isolates),Pythium aphanidermatum(18 isolates) according to the cultural characteristics,morphology,pathogenicity and the rDNA-ITS sequences of the isolates.The results of the pathogenicity of these pathogens revealed that P.sojae,F.oxysporum and F.solani isolates showed strong pathogenicity to soybean roots by artifical infection,leading to disease incidences by 100%、97.8% and 95.6%,respectively,but R.solani and P.aphanidermatum isolates showed weak pathogenicity.
Keywords:soybean root rot  pathogen identification  ITS  pathogenicity
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