‘Sorbonne’百合器官离体培养再生鳞茎的研究

通过对东方百合索蚌鳞片、鳞片叶分段诱导再生鳞茎以及鳞茎膨大的研究,建立了快繁体系。结果表明:诱导鳞片及鳞片叶产生再生鳞茎的最佳培养基都为MS+6-BA0.2(单位:mg·L-1,下同)+NAA0.2+2,4-D0.1;三种放置鳞片的方式对诱导生成的鳞茎的数量、鲜重、诱导率影响不显著;鳞片叶基部切段再生鳞茎的诱导率最高,其次为中部切段,由下向上再生鳞茎的诱导率逐渐降低;最适合诱导再生鳞茎的蔗糖浓度为30~60 g·L-1,黑暗条件下促使鳞茎膨大的蔗糖浓度以低于90 g·L-1为宜,散射光条件下促使鳞茎膨大的蔗糖浓度以低于120 g·L-1为宜。

Regeneration of Bulblets from Tissue Culture of Oriental Hybrid Lilium,Sorbonne

An efficient tissue culture was developed using the bulb scale,scale′s leave segments and bulblet enlargement of the Oriental Hybrid Lilium,"Sorborne".Results showed that(a) the appropriate induction medium for regeneration of the bulblet from the bulb scale and scale′s leave segments was MS+ 0.2 mg·L-1 6-BA+NAA 0.2 mg·L-1 +0.1 mg·L-1 2,4-D;(b) there was no significant difference in quantity,induced rate and fresh weight of the regenerated bulblets resulting from the 3 putting methods;(c) the induction rate of the regenerated bulblet of scale’s leave segments was the highest in the basal,followed by the middle section,and gradually decreased from bottom to top;(d) 30-60 g·L-1 was the optimal sucrose concentration for inducing the bulblet regeneration;(e) for bulblet enlargement,the sucrose concentration should be lower than 90 g·L-1 when treated in the dark,and 120 g·L-1 when exposed under scattered light.