| 部分中国野生双孢蘑菇的DNA鉴定和遗传分析 |
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| 引用本文： | 蔡志欣,陈美元,廖剑华,蔡丹凤,李洪荣,郭仲杰,王泽生.部分中国野生双孢蘑菇的DNA鉴定和遗传分析[J].福建农业学报,2011,26(2):248-253. |
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| 作者姓名： | 蔡志欣 陈美元 廖剑华 蔡丹凤 李洪荣 郭仲杰 王泽生 |
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| 作者单位： | 福建省农业科学院食用菌研究所; |
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| 基金项目： | 福建省科技计划重大专项专题(2008SZ0002-M-1) |
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| 摘 要： | 对41株经同工酶初步鉴定的中国野生双孢蘑菇菌株及作为对照的8株国内外栽培菌株和14株国外野生菌株进行基因组DNA的SRAP和ISSR分析,并对部分菌株进行ITS序列的扩增与序列比对.结果发现中国野生双孢蘑菇与对照菌株的ITS序列同源性高达99%以上,为它们的进一步鉴定提供了DNA水平的依据,也表明同工酶鉴定和ITS序列...
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| 关 键 词： | 食用菌 野生菌株 ITS SRAP ISSR |
DNA Identification and Genetic Analysis on Wild Agaricus bisporus Strains in China |
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| CAI Zhi-xin,CHEN Mei-yuan,LIAO Jian-hua,CAI Dan-feng,LI Hong-rong,GUO Zhong-jie,WANG Ze-sheng.DNA Identification and Genetic Analysis on Wild Agaricus bisporus Strains in China[J].Fujian Journal of Agricultural Sciences,2011,26(2):248-253. |
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| Authors: | CAI Zhi-xin CHEN Mei-yuan LIAO Jian-hua CAI Dan-feng LI Hong-rong GUO Zhong-jie WANG Ze-sheng |
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| Institution: | CAI Zhi-xin,CHEN Mei-yuan,LIAO Jian-hua,CAI Dan-feng,LI Hong-rong,GUO Zhong-jie,WANG Ze-sheng(Edible Fungi Institute of Fujian Academy of Agricultural Sciences Fuzhou,Fujian 350014,China) |
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| Abstract: | Genomic DNAs of 41 wild Agaricus bisporus strains identified by esterase isozymes analysis,along with 8 cultivation strains and 14 foreign wild strains as controls,were extracted for SRAP and ISSR analyses.Internal transcribed spacer(ITS) sequences of partial strains were amplified and the sequences were aligned.The results showed that the homology of ITS sequences from the wild A.bisporus and the control strains surpassed 99%.Thus,ITS sequence could be used as DNA-level evidences for further identification... |
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| Keywords: | edible fungus wild mushroom ITS SRAP ISSR |
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