鸭盲肠杯叶吸虫病的人工感染试验

为研究鸭盲肠肿大坏死症的病因,对38只20日龄肉鸭分别予以饲喂患盲肠肿大坏死症的病鸭内脏混合物、发病地的泥鳅以及从泥鳅体内分离出的囊蚴,进行人工感染试验。结果显示:泥鳅饲喂组和500个囊蚴饲喂组均出现典型的盲肠肿大坏死症症状,100个囊蚴饲喂组只有轻微的症状和盲肠病变;从泥鳅饲喂组、500个囊蚴饲喂组和100个囊蚴饲喂组病变盲肠内均检出盲肠杯叶吸虫,平均数量分别39、61.7和11.8条.只-1,3组均检出虫卵;而病鸭内脏饲喂组不发病,亦未检出盲肠杯叶虫。试验结果表明盲肠杯叶吸虫是鸭盲肠肿大坏死症的病原,病例发生地水田中的泥鳅是盲肠杯叶吸虫的第二中间宿主。

The Artificial Infection of Duck's Cecum Enlargement and Necrosis Syndrome

For the etiological researching of Duck’s Cecum Enlargement and Necrosis Syndrome(DCENS),38 20-days old ducks were fed with organ mixture from duck infected with DCENS,loach from the onset area and metacercariae isolated from loach respectively.Those fed with loachs and with 500 metacercariae showed typical symptoms of DCENS;but those fed with 100 metacercariae showed only mild symptoms and cecal lesion.The average number of Cyathocotyle caecumalis sp.nov isolated from the duck cecum of the group fed with loachs,500 metacercariae and 100 metacercariae were 39,61.7and 11.8,and eggs were checked out from all the three groups;while the group fed with organ mixture showed none symptom and Cyathocotyle caecumalis sp.nov detection.The results suggested that C.caecumalis sp.nov is the only pathogen of DSCEN.The loach from the paddy field of the onset area is the second intermediate host of C.caecumalis sp.nov.