蛋白核小球藻多糖的酶解辅助提取及抗氧化活性

利用纤维素酶对蛋白核小球藻多糖进行辅助提取,并在单因素的基础上,通过响应面试验对提取时间、提取温度、料液比和酶浓度等4个因素进行优化。结果表明:酶法辅助提取蛋白核小球藻多糖的最佳条件为:提取时间为2h、提取温度为93℃、料液比为1∶30和酶浓度为2%,在该条件下多糖提取率可达6.13%,实际为(5.87±0.24)%。同时研究其对DPPH自由基、超氧阴离子自由基及羟基自由基的清除能力,结果表明蛋白核小球藻多糖能有效清除超氧阴离子自由基及羟基自由基。

Hot-water Extraction of Polysaccharides from Chlorella pyrenoidosa with Cellulase

Cellulase was added to the hot water extraction of polysaccharides from Chlorella pyrenoidosa in an attempt to facilitate the process .The single factor test and the response surface methodology were used to optimize the conditions .It was found that whenthe substrate :liquid ratio of 1∶30 (g?mL-1 ) with 2% (w/w) of cellulase addition was extracted at 93℃ for 2 h , an expected 6.13% yield on polysaccharidescould be obtained (the actual yield was 5.87 0.24% ) . Subsequently ,the polysaccharides obtained was tested for its scavenging activities on DPPH , O2- ,and hydroxyl free radicals . The results confirmed itsantioxidant abilities agains O 2- and hydroxyl free radicals .