| 丝瓜总酚提取和测定方法的优化 |
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| 引用本文： | 朱海生,庄尹宏,刘建汀,王彬,陈敏氡,张前荣,温庆放,李大忠,薛珠政.丝瓜总酚提取和测定方法的优化[J].福建农业学报,2016(11):1204-1209. |
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| 作者姓名： | 朱海生 庄尹宏 刘建汀 王彬 陈敏氡 张前荣 温庆放 李大忠 薛珠政 |
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| 作者单位： | 福建省农业科学院作物研究所，福建 福州 350013; 福建省农业科学院蔬菜研究中心，福建 福州 350013; 福建省蔬菜工程技术研究中心，福建 福州 350013 |
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| 基金项目： | 福建省农业科学院科技创新团队 PI 项目(2016PI-40)，福建省自然科学基金项目(2015J01118)，国家大宗蔬菜产业体系福州试验站(CARS-25-G-20) |
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| 摘 要： | 为建立简单、稳定的丝瓜总酚提取和测定体系,对丝瓜果肉总酚的超声波辅助提取和福林-酚比色测定方法进行优化。丝瓜果肉与80%乙醇在比例为1∶10、40℃下超声波提取30min,总酚的提取效果较好。在测定波长750nm、0.5mol·L~(-1) Na2CO3体积3mL、0.5mol·L~(-1)福林-酚体积1.5mL、反应温度30℃、反应时间60min时,总酚含量与吸光度呈良好的线性关系:Y=0.6279 X+0.0273(R2=0.9947),说明此方法能够简便、准确、快速的测定丝瓜总酚含量。
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| 关 键 词： | 丝瓜 总酚 超声波 福林-酚 |
Extraction and Determination of Total Phenol in Luffa (Luffa cylindrical Roem.) |
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| Abstract: | To establish a simple and reliable extraction and measurement method for the total phenol content in luffa (Luffa cylindrical Roem),ultrasound and Folin-Ciocaleu colorimetry were applied and operational procedures optimized.Phenols could be optimally extracted from the luffa mesocarp when it was mixed ultrasonically with 80%ethanol in 1 ∶ 10 ratio at 40 ℃ for 30 min.The total phenolic content had a linear correlation (Y = 0.6279X +0.0273;R 2 = 0.9947 )with the optical absorbance at 750 nm when the solution was made with 3 mL of 0.5 mol·L-1 Na2 CO3 and 1.5 mL of 0.5 mol·L-1 Folin-Ciocalteu to react with the sample for 60 min at 30℃.The newly developed extraction and subsequent measurement procedures were simple,rapid,sensitive and accurate.It was considered adequate for the determination of total phenol in luffa. |
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| Keywords: | luffa total phenol ultrasound folin-ciocalteu |
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