养猪发酵床垫料微生物类群结构特性分析

采用定点取样、平板分离的方法,分析养猪发酵床垫料微生物的结构特性。结果表明,在30个样点5次取样过程中,微生物含量总体为细菌放线菌真菌。细菌含量变化幅度最大(33.72~197.23)×10~5cfu·g~(-1)],真菌含量变化相对较小(7.87~48.64)×10~3cfu·g~(-1)],而放线菌含量变化稳定(20.14~33.18)×10~4cfu·g~(-1)]。细菌含量类群Ⅰ(大于70.00×10~5cfu·g~(-1))平均9.2个样点、占总样点的28.67%;类群Ⅲ(小于30.00×10~5 cfu·g~(-1))平均为8.6个样点,占总样点的30.67%;类群Ⅱ(30.00~70.00)×10~5cfu·g~(-1)]平均为12.2个样点,所占比例最大,为40.67%。真菌类群Ⅰ(大于70.00×10~3cfu·g~(-1))平均仅3.8个样点,所占比例最低,为12.67%;类群Ⅱ(30.00~70.00)×10~3cfu·g~(-1)]平均为6.4个样点,所占比例为21.33%;类群Ⅲ(小于30.00×10~3cfu·g~(-1))平均19.8个样点,所占比例高达66.00%。放线菌3种类群所占比例与真菌类似,类群Ⅰ(大于70.00×10~4cfu·g~(-1))平均仅1.4个样点,所占比例最低,仅为4.67%;类群Ⅱ(30.00~70.00)×10~4cfu·g~(-1)]平均8.4个样点,所占比例为28.00%;类群Ⅲ(小于30.00×10~4cfu·g~(-1))平均20.2个样点,所占比例高达67.33%。

Analysis on Spatial Distribution of Microbes Population in the Fermentation Bed

Spatial distribution of microbes in the fermentation bed was analyzed by sampling and culturing,the results indicated that the amount of bacterium was highest,the second was actinomycete,the amount of fungi was lowest.In all of the samples,amplitude of variation of bacterial content was the biggest with (33.72~197.23)× 105 cfu·g-1 ,fungal content changes are relatively small with (7.87 ~48.64) ×103 cfu·g-1 ,and Actinomyces content was stability with (20.14~33.18)×104 cfu·g-1 .GroupⅠ (more than 70.00×105 cfu·g-1 )of bacteria content was averaged 9.2 sample points,accounting for 28.67% of the total sample;group III (less than 30.00× 105 cfu·g-1 )was averaged 8.6 points,accounting for 30.67% of the total sample.Group II (30.00 ~70.00)× 105 cfu·g-1 was averaged 12.2 points,accounted for the largest proportion of 40.67%.Fungal group I (more than 70.00×103 cfu·g-1 )was averaged only 3.8 points,the lowest proportion for 12.67%,Group II (30.00~70.00) ×103 cfu·g-1 was averaged 6.4 points,the proportion was 21.33%,group III (less than 30.00 ×103 cfu·g-1 ) was averaged 19.8 points,a whopping 66.00%.The proportion of actinomyces was similar to type of fungi,group I (more than 70.00 × 104 cfu · g-1 )was averaged just1.4 sample points,the lowest proportion,only 4.67%. Group II (30.00~70.00)×104 cfu·g-1 was averaged 8.4 points and percentage 28%,Group III (less than 30.00 ×104 cfu·g-1 )was averaged 20.2 sample points,a whopping 67.33%.