淡水砂梨离体快繁技术研究

为建立淡水砂梨离体培养体系,以淡水砂梨品系香水梨为试验材料,以MS为基本培养基,研究生长调节剂不同组合和浓度对腋芽诱导、增殖培养的影响,以及不同生长调节剂的种类和浓度对生根培养的影响。结果表明,香水梨腋芽诱导的最佳培养基配方为MS+6-BA 1.0 mg·L~(-1)+NAA 0.1 mg·L~(-1),诱导率为71.7%;增殖最适宜的培养基配方为MS+6-BA 3.0mg·L~(-1)+NAA 0.20mg·L~(-1),增殖系数为3.2;生根培养基配方为1/2MS+IBA 0.3mg·L~(-1),香水梨生根率为63.3%。研究表明MS为基本培养基添加生长调节剂适于淡水砂梨的离体快繁。

In Vitro Propagation of Dan-Shui Pear

To establish an in vitro culture system for propagating Dan-Shui pear,Xiangshui pear was used on Murashige and Skoog medium (MS) as the basal medium for the experimentation.Conditions of the tissue culture,such as hormone combination and concentration on explant induction and proliferation culture,as well as hormone types and concentrations on rooting culture,were studied.The optimal combination for the axillary bud induction was found to be MS+ 6-BA1.0mg· L 1+NAA0.1mg· L-1 that yielded a germination rate of 71.7 ％.For the bud proliferation,it was MS+6-BA 3.0 mg· L-1 + NAA 0.20 mg · L-1 to deliver a multiplication coefficient of 3.2.For the optimal rooting performance,the combination of 1/2 MS+IBA 0.3 mg · L-1 to result in a rooting rate of 63.3％ was selected.Thus,addition of the growth regulators to MS medium benefitted the in vitro propagation of Dan-Shui pear.