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| 猪传染性胃肠炎病毒福建株M基因的克隆与生物信息学分析 | |
| 引用本文： | 朱晓琳,王劭,陈少莺,林锋强,程晓霞,陈仕龙,朱小丽,李兆龙.猪传染性胃肠炎病毒福建株M基因的克隆与生物信息学分析[J].福建农业学报,2011,26(6):925-929. |
| 作者姓名： | 朱晓琳 王劭 陈少莺 林锋强 程晓霞 陈仕龙 朱小丽 李兆龙 |
| 作者单位： | 1. 福建省农业科学院畜牧兽医研究所,福建福州 350012;福建农林大学动物科学学院,福建 福州 350002 2. 福建省农业科学院畜牧兽医研究所,福建福州 350012;福建省畜禽疫病防治工程技术研究中心,福建福州350013 3. 福建省农业科学院畜牧兽医研究所,福建福州,350012 |
| 基金项目： | 福建省财政专项——福建省农业科学院创新团队项目(STIF-Y02);福建省科技计划项目——省属公益类科研院所基本科研专项(2010R1025-1) |
| 摘 要： | 对猪传染性胃肠炎病毒(porcine transmissible gastroenteritis virus,TGEV)福建分离株(TGEV-FJ 株)M基因进行克隆、测序,并利用生物信息学技术分析TGEV-FJ株M蛋白基因的同源性、信号肽、糖基化位点、磷酸化位点及其二级结构.结果显示,成功扩增出M基因完整目的片段(7... |
| 关 键 词： | 猪传染性胃肠炎病毒 M基因 克隆 生物信息学 |
Cloning and Bioinformatics Analysis of M Gene of Porcine Transmissible Gastroenteritis Virus Fujian Strain |
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| ZHU Xiao-lin , WANG Shao , CHEN Shao-ying , LIN Feng-qiang , CHENG Xiao-xia , CHEN Shi-long , ZHU Xiao-li , LI Zhao-long.Cloning and Bioinformatics Analysis of M Gene of Porcine Transmissible Gastroenteritis Virus Fujian Strain[J].Fujian Journal of Agricultural Sciences,2011,26(6):925-929. | |
| Authors: | ZHU Xiao-lin WANG Shao CHEN Shao-ying LIN Feng-qiang CHENG Xiao-xia CHEN Shi-long ZHU Xiao-li LI Zhao-long |
| Institution: | 1(1.Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agriculture Sciences, Fuzhou,Fujian 350013,China;2 Fujian Animal Diseases Control Technology Development Center, Fuzhou,Fujian 350013,China;3.College of Animal Science,Fujian Agriculture and Forestry University, Fuzhou,Fujian 350002,China) |
| Abstract: | In this study,we report the cloning and sequencing of M gene of porcine transmissible gastroenteritis virus Fujian strain(TGEV-FJ),and analyze the homology,signal peptide,glycosylation sites,phosphorylation sites,secondary structure of M protein by using bioinformatics software.The complete M gene in TGEV-FJ strain was successfully amplified which has 789 bp,encoding 262 amino acids and then constructed into the recombinant plasmid pGEM-T-M.Sequential analysis and phylogenetic studies demonstrated that the TGEV M gene has highly conservative and consanguineous between TGEV-FJ strain and HN2002 strain.The prediction results showed that M protein in TGEV-FJ strain had a signal peptide cleavage site(ACG16-17ER),and 3 potential N-glycosylation sites,6 serine phosphorylation sites,1 threonine phosphorylation sites and 5 tyrosine phosphorylation sites.The secondary structure of M protein prediction showed that alpha helix,beta sheet and random coil were 11.59%,44.93% and 43.48%,respectively.The results of the present study would lay some basic molecular biological foundation for prokaryotic expression of M gene in TGEV-FJ strain,which would subsequently facilitate further research for molecular diagnosis and gene engineering vaccines development. |
| Keywords: | TGEV M gene cloning bioinformatics analysis |
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