| 发酵鱼粉中寡肽检测技术研究 |
| |
| 引用本文： | 罗钦,陈人弼,宋永康.发酵鱼粉中寡肽检测技术研究[J].福建农业学报,2008,23(3). |
| |
| 作者姓名： | 罗钦 陈人弼 宋永康 |
| |
| 作者单位： | 福建省农业科学院中心实验室,福建,福州,350003 |
| |
| 基金项目： | 福建省农科院青年科技人才创新基金项目(2006) |
| |
| 摘 要： | 用单宁酸作为发酵鱼粉中水溶性蛋白质的沉淀剂，经飞行时间质谱仪测得滤液中蛋白质分子量在2047．98Da以下。用双缩脲反应法可以测出发酵鱼粉中水溶性总肽的含量，用凯氏定氮法可以测出被沉淀的多肽的含量，从而可以计算出发酵鱼粉中寡肽的含量。用该技术测得2546Da和1500Da的低分子量蛋白的回收率分别为92．42％和5．91％。
|
| 关 键 词： | 寡肽 单宁 双缩脲反应法 凯氏定氮法 检测技术 |
Determination of oligopeptide content in fermented fishmeal |
| |
| LUO Qin,CHEN Ren-bi,SONG Yong-kang.Determination of oligopeptide content in fermented fishmeal[J].Fujian Journal of Agricultural Sciences,2008,23(3). |
| |
| Authors: | LUO Qin CHEN Ren-bi SONG Yong-kang |
| |
| Abstract: | Tannic acid was used to precipitate soluble proteins in fermented fishmeal.Applying a time-of-flight mass spectrometer,the molecular weight of the protein in the filtrate was found to be lower than 2047.98Da.Total solvable peptide content in the fermented fishmeal was determined by using the biuret method,and polypeptide in the precipitate by using Kjeldahl method.Then,the oligopeptide content was calculated.This determination method showed recovery rates for the 2546 Da and 1500 Da low molecular weight pro... |
| |
| Keywords: | Oligopeptides tannin biuret method Kjeldahl method determination methodology |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
