叶底红花芽分化期叶片内源激素的动态变化

应用高效液相色谱法(HPLC)测定了叶底红花芽分化期叶片中内源激素吲哚乙酸(IAA)、赤霉素(GA3)、玉米素(ZR)和脱落酸(ABA)等4种内源激素含量的动态变化。结果显示,这4种激素均有明显的变化规律,其中GA3和ZR含量在花芽分化基本完成时均达到高峰,相反IAA含量处于低谷;ABA含量在花芽分化中期达到高峰,而此时GA3和ZR含量处于低谷;内源激素含量峰、谷时间和叶底红花芽分化的完成时间相一致;4种激素含量的变化均达到极显著差异。研究表明这几种激素均与叶底红花芽分化密切相关,高水平的ABA/IAA、ZR/IAA、ZR/GA3有利于花芽分化,高水平的ABA/GA3有利于花芽孕育。

Dynamic Changes of Endogenous Hormone in Phyllagathis fordii Leaf during Flower Bud Differentiation

Changes on the hormones in the leaves of Phyllagathis fordii during its flower bud differentiation stage weremonitored.Contents of 4 endogenous hormones,i.e.,IAA,GA3,ZR and ABA,were determined by using HPLC.The results showed that,at the completion of flower bud differentiation,the contents of GA3 and ZR peaked,while IAA reached its lowest level in the leaves.In the mid of the differentiation stage,ABA maximized while GA3 and ZR were low.The changes on these endogenous hormones highly significantly related to the flower bud differentiation.It was found that high level of ABA/IAA,ZR/IAA,and ZR/GA3ratios in the leaves benefited the differentiation,whereas,high ABA/GA3favored the induction of theflower buds.