甘薯蔓割病抗性相关SRAP标记的获得

以高抗甘薯蔓割病品种金山57和高感蔓割病甘薯品种新种花为亲本进行杂交,获得F1分离群体,对F1群体进行蔓割病抗性鉴定,从中选出7个高抗和7个高感蔓割病株系构建抗、感池。以抗、感池基因组DNA为模板,用276对SRAP引物组合对其扩增,从中筛选出98对具有多态性的引物组合,再以抗感亲本加抗感池基因组DNA为模板筛选,从98份SRAP引物组合中筛选出33对多态性较好的引物组合。后通过用一组小群体(6个高抗株系和6个高感株系)进一步筛选,最终获得一对与甘薯抗蔓割病连锁的SRAP引物组合a12b8,经过抗感亲本及F1代的进一步验证,认为该标记与甘薯抗蔓割病基因相关。

SRAP Marker Associated with Fusarium-wilt Resistance Gene in Sweet Potato

Fusarium wilt (F.W) disease-resistant molecular markers can be used to screen disease-resistant lines in early planting stage of sweet potato, which can also greatly improve the efficiency of breeding for sweet potato disease-resistance.The F1 progenies were obtained from the cross of sweet potato c.v. Jinshan57, which is higly resistant to Fusarium wilt (F.W), and c.v. Xinzhonghua, which is highly susceptible to F.W. After disease-resistant identification, 7 highly disease-resistant and 7 highly disease-susceptible lines were selected from F1 progenies and were used to establish the resistant and susceptible DNA pools. The genomic DNA of the resistant and susceptible pools were PCR-amplified respectively with 276 pairs of SRAP primers，and 98 pairs of primer generated polymorphic bands between the disease-resistant and susceptible pools. Through further screening using a group of small population and their parents, we got a SRAP marker named a12b8, which would be linked to the resistance of F.W. in sweet potato. This marker would be used for identifying the resistance to F.W in the early growth stage of sweet potato and also would be utilized for improving the efficiency of F.W disease-resistant breeding by molecular marker assisted selection for sweet potato.