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我国斑鳜不同群体mtDNA控制区序列的遗传变异
引用本文:王伟伟,赵金良,李思发.我国斑鳜不同群体mtDNA控制区序列的遗传变异[J].上海水产大学学报,2006,15(4):398-402.
作者姓名:王伟伟  赵金良  李思发
作者单位:上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海200090
基金项目:上海市教委资助项目;上海市重点学科建设项目
摘    要:采用PCR技术扩增了鸭绿江、长江、钱塘江、闽江、西江5个群体36尾斑鳜(Siniperca scherzeri Steindachner)mtDNA控制区的基因序列。在长度818bp片断序列中共检测到112个多态性核苷酸位点,具有20种单倍型,不同地理群体的单倍型表型不同,鸭绿江、钱塘江群体中含有多个特异性的核苷酸位点。用MEGA3.0构建了NJ分子树,鸭绿江、长江、钱塘江、西江群体内的个体均能单独聚成群,而闽江群体未能单独成群,个体分别与长江、西江群体聚在一起。这些表明我国斑鳜不同群体间有较明显的遗传分化。

关 键 词:斑鳜  群体  控制区  序列
文章编号:1004-7271(2006)04-0398-05
收稿时间:2005-11-10
修稿时间:2005年11月10

Genetic variation of the mitochondrial DNA control region among 5 populations of Siniperca scherzeri Steindachner in China
WANG Wei-wei,ZHAO Jin-liang,LI Si-fa.Genetic variation of the mitochondrial DNA control region among 5 populations of Siniperca scherzeri Steindachner in China[J].Journal of Shanghai Fisheries University,2006,15(4):398-402.
Authors:WANG Wei-wei  ZHAO Jin-liang  LI Si-fa
Institution:Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecosystem Certificated by the Ministry of Agriculture, Shanghai Fisheries University, Shanghai 200090, China
Abstract:The mitochondrial DNA control regions of Siniperca scherzeri Steindachner collected from the Yalujiang River, Yangtze River, Qiantangjiang River, Minjiang River and Xijiang River were amplified with PCR technique and sequenced. The result showed 112 nucleotide sites were variable among 818 bp length of homologous sequence and 20 haplotypes were found in all 36 individuals. Each population owned the different haplotypes, some population specific sites were also found in the populations from the Yalujiang River and Qiantangjiang River. Molecular tree constructed by NJ method showed all the individuals from the Yalujiang River, Yangtze River, Qiantangjiang River and Xijiang River populations, respectively, could be clustered as one group independently, except that the individuals from the Minjiang River were mixed with those of the Yangtze River and Xijiang River. These showed there were significant genetic differences among the 5 populations of S. scherzeri in China.
Keywords:Siniperca scherzeri population  control region  sequence
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