酵母聚糖和甘氨酸锌对栉孔扇贝血细胞的影响

采用流式细胞术对栉孔扇贝(Chlamys farreri)血细胞类群百分比的变化进行了研究,以探讨酵母聚糖和甘氨酸锌对贝类免疫防御影响的规律。体内注射酵母聚糖和甘氨酸锌后,分别于6 h、12 h、24 h、48 h、96 h和144 h测定其血细胞各类群的比例的变化。根据前向角散射光(FSC)和侧向角散射光(SSC)强度的不同,栉孔扇贝血细胞可明显地分为透明细胞、小颗粒细胞和大颗粒细胞3个类群。注射酵母聚糖后,在6 h、12 h、24h和48 h时,实验组透明细胞占总血细胞的比例显著高于对照组,而实验组小颗粒细胞所占比例在6 h、12 h和24 h时显著低于对照组。注射甘氨酸锌后,在12 h、24 h和48 h时,实验组透明细胞所占比例显著高于对照组,而小颗粒细胞所占比例显著低于对照组。说明酵母聚糖和甘氨酸锌对栉孔扇贝血细胞的分群有明显的影响,可显著刺激透明细胞的数量增多,同时颗粒细胞数量减少。

The effects of glycine-Zn and zymosan on haemocyte in Chlamys farreri

Haemocyte subpopulation percentage variety of Chlamys farreri was studied by light-scatter flow cytometry,and the aim is to determine the role of zymosan and glycine-Zn in the immune response of molluscs.After injection of zymosan and glycine-Zn,the subpopulation percentages variety of the haemocytes were assayed at 6 h,12 h,24 h,48 h,96 h and 144 h.Three haemocyte groups,hyalinocyte,small granulocyte and large granulocyte,were found in the haemolymph of Chlamys farreri by the analysis method of two parameter plots(FSC,Forward Scatter and SSC,Side Scatter).After injection with zymosan,the hyalinocyte percentage in haemocytes of experimental groups were significantly higher than that of control groups at 6 h,12 h,24 h and 48 h.But the small granulocyte percentage were significantly lower than that of control at 6 h,12 h and 24 h.After injection with glycine-Zn,the hyalinocyte percentage in haemocytes was significantly higher than that of control groups at 12 h,24 h and 48 h.However,small granulocyte percentage was significantly lower than that of control groups at 12 h,24 h and 48 h.These results indicated that zymosan and glycine-Zn had great effects on haemocyte subpopulation percentages variety of Chlamys farreri,and they could cause the increase of hyalinocyte and decrease of granulocyte.