| 团头鲂乳酸脱氢酶与苹果酸脱氢酶组织特异性的激光扫描分析 |
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| 引用本文： | 李思发,蔡完其.团头鲂乳酸脱氢酶与苹果酸脱氢酶组织特异性的激光扫描分析[J].上海水产大学学报,1992(Z1). |
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| 作者姓名： | 李思发 蔡完其 |
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| 作者单位： | 上海水产大学水产养殖系 200090
(李思发)，上海水产大学水产养殖系 200090(蔡完其) |
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| 基金项目： | 加拿大国际发展研究中心组织和资助的亚洲鱼类遗传研究网的中国课题~~ |
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| 摘 要： | 用LKB水平电泳仪,4.4%聚丙烯酰胺凝胶,对10—11月龄团头鲂(全长14—16cm)的11种组织——白肌、晶体、脑、脾、肾、性腺、心、肝、鳍、红血球及血清的乳酸脱氢酶和苹果酸脱氢酶作电泳,再用LKB激光光密度计对同工酶的迁移率及活性作定性定量分析。在不同组织中,同工酶的迁移率和活性有明显的组织特异性。
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| 关 键 词： | 团头鲂 同工酶 电泳 激光扫描 组织特异性 迁移率 活性 |
DENSITOMETRIC SCANNING ANALYSIS ON TISSUE SPECIFIC ISOZYMES OF BLUNT SNOUT BREAM (MEGALOBRAMA AMBLYCEPHALA) |
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| LiSi-fa,Cai Wan-qi.DENSITOMETRIC SCANNING ANALYSIS ON TISSUE SPECIFIC ISOZYMES OF BLUNT SNOUT BREAM (MEGALOBRAMA AMBLYCEPHALA)[J].Journal of Shanghai Fisheries University,1992(Z1). |
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| Authors: | LiSi-fa Cai Wan-qi |
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| Institution: | LiSi-fa;Cai Wan-qi Department of Aquaculture. SFU. 200090 |
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| Abstract: | In LKB electrophoresis set with 4.4% polycrylamide gel, the lactate dehydrogenase(LDH) and malate dehydrogenase (MDH) were examined from 11 tissues-white muscle, eye lens,brain, liver, kidney, gonad, heart, spleen, fin, red cell and serum of blunt snout bream. Qualitativeand quantitative analysis on mobility and activity were conducted by a LKB Ultorscan laser de-nsitometer. The activity and mobility of isozymes in different tissues showed a significant tissuespecificity. |
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| Keywords: | blunt snout bream (Megalobrama amblycephala) isozyme electrophoresis laser scanning tissue speificity mobility activity |
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