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长江口日本鳗鲡群体遗传多样性RAPD初步分析
引用本文:龚小玲,李思发,王成辉.长江口日本鳗鲡群体遗传多样性RAPD初步分析[J].上海水产大学学报,2007,16(3):201-206.
作者姓名:龚小玲  李思发  王成辉
作者单位:上海水产大学生命科学与技术学院、上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海200090
基金项目:上海市科技攻关项目;上海市重点学科建设项目
摘    要:对2005年采集自长江口地区4个采样点、5批、共10尾性成熟前成鳗、82尾玻璃鳗的日本鳗鲡样本的遗传多样性进行了RAPD分析。在51条随机引物中筛选出具有稳定扩增、条带清晰的多态性引物16条,16条引物共检测到132个位点,其中多态位点114个(86.36%)。五个采样群体各自的平均杂合度(Hc)在0.2053~0.3150之间,平均基因多样性指数(Ho)在0.2974~0.4526之间;采样群体两两间的遗传分化指数几,在0.0508~0.1524之间。群体内遗传变异和群体间遗传变异对群体总遗传变异的贡献率分别为79.18%和20.82%;Nei的遗传距离在0.0593~0.1425之间;在玻璃鳗采样群体间未发现遗传差异同采样地点及月份有何显著关系,显示它们可能同属一个大混合群体。而遗传距离、遗传分化指数及NJ树分析结果一致表明:成鳗和玻璃鳗之间可能存在相当的遗传距离和遗传分化。

关 键 词:日本鳗鲡  长江口  遗传多样性  遗传距离
文章编号:1004-7271(2007)03-0201-06
收稿时间:2007-01-23
修稿时间:2007-01-23

Primary study on the genetic diversity of Japanese eel (Anguilla japonica) from the Yangtze River Estuary by RAPD analysis
GONG Xiao-ling,LI Si-fa,WANG Cheng-hui.Primary study on the genetic diversity of Japanese eel (Anguilla japonica) from the Yangtze River Estuary by RAPD analysis[J].Journal of Shanghai Fisheries University,2007,16(3):201-206.
Authors:GONG Xiao-ling  LI Si-fa  WANG Cheng-hui
Institution:College of Aqua-life Science and Technology, Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecosystem Certificated by the Ministry of Agriculture, Shanghai Fisheries University, Shanghai 200090, China
Abstract:Random amplified polymorphic DNA method was used to investigate the genetic diversity of Anguilla japonica including five samplings collected from 4 locations,totally 92 individuals(10 premature adult eels,82 glass eels) in 2005 from the Yangtze River Estuary.51 RAPD primers were synthesized and 16 primers were selected and used.A total of 132 loci were amplified,of which 114 loci(86.36%) were polymorphic.Average heterozygosity(Hc) and genetic diversity index(Ho) of five samplings by Shannon index varied form 0.205 3 to 0.315 0,and 0.297 4 to 0.452 6 respectively.Fixation Index FST between two samplings varied from 0.050 8 to 0.152 4.Partition of total genetic variation indicated that 79.12% was contributed within populations and 20.82% among samplings.The Nei's genetic distances of five samplings varied form 0.059 3 to 0.142 5.There is no significant relationship between the genetic variation of samplings of glass eel and the sampling time or the sampling area.It indicates the all studied glass eels might be originated from one big complex population.But the results from genetic distance,Fixation Index FSt and NJ tree all showed,a rather high difference in genetic distance and genetic diversity could be exist between the premature adult eels and glass eels.
Keywords:RAPD
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