| 我国近海中国对虾种群遗传差异的RAPD分析 |
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| 引用本文： | 邱高峰,常林瑞.我国近海中国对虾种群遗传差异的RAPD分析[J].上海水产大学学报,2001,10(1):1-5. |
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| 作者姓名： | 邱高峰 常林瑞 |
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| 作者单位： | [1]上海水产大学渔业学院，上海200090 [2]烟台师范学院生物学系，山东烟台264025 |
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| 基金项目： | 上海市教委高校跨世纪人才培养基金--"曙光计划”项目(编号:96SG18)的部分内容 |
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| 摘 要： | 应用随机扩增多态DNA（RAPD）技术对取自我国近海烟台、长岛、青岛、宁波等4个地方的20只中国对虾进行了种群内和种群间遗传差异分析。在使用的50个随机引物中，有9引物扩增出条带清晰的多态性片段。烟台、长岛、青岛、宁波等4个地理种群内的遗传距离分别为0．0154、0．0370、0．0462、0．0550，种群之间的遗传距离分别为0．0793、0．1139、0．0988、0．1485、0．0880、0．1260。UPGMA和NJ法构建的种群间分子系统树基本一致，结果表明不同地理种群这间存在一定程度的遗传差异。
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| 关 键 词： | 中国对虾 种群 遗传差异 遗传距离 RAPD分析 |
| 文章编号： | 1004-7271(2001)01-0001-05 |
| 修稿时间： | 2000年8月29日 |
Population genetic variation of Chinese shrimp Penaeus chinensis along the coast
of China assessed by random amplified polymorphic DNA (RAPD) |
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| QIU Gao feng ,CHANG Lin rui.Population genetic variation of Chinese shrimp Penaeus chinensis along the coast
of China assessed by random amplified polymorphic DNA (RAPD)[J].Journal of Shanghai Fisheries University,2001,10(1):1-5. |
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| Authors: | QIU Gao feng CHANG Lin rui |
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| Institution: | QIU Gao feng 1,CHANG Lin rui 2 |
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| Abstract: | Random amplified polymorphic DNA(RAPD) markers were used to detected 4 populations genetic variation of 20 Chinese shrimps (Penaeus chinensis) along the coast of China, 5 from each of samples caught off Yantai, Changdao, Qingdao, and also for 5 individuals from a cultured sample collected off Ningbo. 9 primers screen from 50 random primers generated 54 RAPD bands, among which 36 were varied. The intra population genetic distances among those 4 populations (Yantai, Changdao, Qingdao, Ningbo) were 0.0154,0.0370,0.0462,0.0550, respectively. The inter population genetic distances among them were 0.0793,0.1139,0.0988,0.1485,0.0880,0.1260, respectively. Molecular phylogenetic tree constructed by UPGMA and NJ method suggested that there were some genetic variation among different geographic population. |
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| Keywords: | Penaeus chinensis random amplified polymorphic DNA population genetic variation |
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