| 麻醉剂MS-222对金鱼体内AKP、CAT和ACP活性的影响 |
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| 引用本文： | 刘伟,陈再忠.麻醉剂MS-222对金鱼体内AKP、CAT和ACP活性的影响[J].上海水产大学学报,2010(3). |
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| 作者姓名： | 刘伟 陈再忠 |
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| 作者单位： | 上海海洋大学水产与生命学院; |
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| 基金项目： | 上海市教委项目(06-415); 上海市重点学科建设项目(Y1101) |
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| 摘 要： | 在实验室条件下,采用4个浓度0mg/L、30mg/L、60mg/L和90mg/L,分析了麻醉剂MS-222对金鱼鳃、肝脏和肌肉组织中碱性磷酸酶(AKP)、酸性磷酸酶(ACP)和过氧化氢酶(CAT)活性的影响。试验结果表明:ACP明显出现一个先增后降的趋势,呈抛物线状;在低浓度(≤60mg/L)下,酶活性在各组织中呈现一定的稳定状态,而当麻醉剂浓度升高和麻醉时间增长,酶活性逐渐下降。AKP在低浓度麻醉剂溶液中,酶活性有明显的增加,随着浓度的升高(≥60mg/L)和时间的增长,酶活性呈抑制状态。而在肌肉中这种情况并不明显。CAT在一定阶段(30mg/L～60mg/L)呈现稳定的状态、而后抑制逐渐明显,当麻醉剂MS-222浓度达到一定量(≥60mg/L)以后,对鱼体的刺激就不再增加了,酶活性也相对处于较稳定的状态;当麻醉剂随着时间的增长和浓度的增大时,在鱼体内富集的麻醉剂破坏鱼体内的过氧化氢酶的分子构象,使酶体失去活性。
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| 关 键 词： | 金鱼 麻醉剂MS-222 酸性磷酸酶 碱性磷酸酶 过氧化氢酶 |
Effects of the anesthetic MS-222 on the AKP,CAT and ACP activities in goldfish |
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| LIU Wei,CHEN Zai-zhong.Effects of the anesthetic MS-222 on the AKP,CAT and ACP activities in goldfish[J].Journal of Shanghai Fisheries University,2010(3). |
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| Authors: | LIU Wei CHEN Zai-zhong |
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| Institution: | LIU Wei,CHEN Zai-zhong(College of Fisheries , Life Science,Shanghai Ocean University 201306,China) |
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| Abstract: | The effect of the anesthetic MS-222 on the activities of alkaline phosphatase(AKP),catalase(CAT)and acid phosphatase(ACP)in goldfish was analyzed under laboratory conditions.Four concentrations 0 mg/L,30 mg/L,60 mg/L and 90 mg/L of MS-222 were tested.The results showed that the concentration of MS-222 affected the activities of AKP,CAT and ACP in the gill,liver and muscle of goldfish.The results show that the activity of alkaline phosphatase is first increased and then decreased,shows a parabola curve which... |
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| Keywords: | goldfish anesthetic MS-222 alkaline phosphatase catalase acid phosphatase |
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