| 氨氮对凡纳滨对虾稚虾的急性毒性作用 |
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| 引用本文： | 魏俊利,孙金生,通信李翔,马文婷,耿绪云,徐赟霞,李晶晶.氨氮对凡纳滨对虾稚虾的急性毒性作用[J].天津农学院学报,2012(3):58-61. |
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| 作者姓名： | 魏俊利 孙金生 通信李翔 马文婷 耿绪云 徐赟霞 李晶晶 |
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| 作者单位： | 1. 天津市水生动物疫病预防控制中心,天津 300221
2. 天津师范大学 细胞和分子调控重点实验室,天津 300387 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)项目“对虾白斑综合征防治的免疫学途径和关键技术原理”(2012CB114405) |
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| 摘 要： | 实验研究了氨氮对体长3．2cm、体质量0．356g（n=10）与体长1．6cm、体质量0．036g（n=15）两种规格凡纳滨对虾（Litopenaeus vannamei）稚虾的急性毒性效应。血氨和碱性磷酸酶活性的测定结果显示，血氨随氨氮浓度的升高而升高，碱性磷酸酶（AKP）活性随氨氮的升高而下降。急性毒性实验结果表明，体长3．2cm稚虾的游离态氨24hLG。为2．068mg／L、48hLC5。为1．654mg／L、96hLG。为1．395mg／L，生存安全浓度sc为0．3969mg／L；体长1．6em稚虾的游离态氨24hLGo为1．577mg／L、48hLG0为1．042mg／L、96hL氏为1．012mg／L，生存安全浓度Sc为0．2065mg／L。
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| 关 键 词： | 凡纳滨对虾稚虾 碱性磷酸酶 血氨 游离态氨 半数致死浓度 生存安全浓度 |
Acute Toxic Effects of Ammonia Nitrogen on Litopenaeus vannamei |
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| WEI Jun-li,SUN Jin-sheng,Corresponding Author,LI Xiang,MA Wen-ting,GENG Xu-yun,XU Yun-xia,LI Jing-jing.Acute Toxic Effects of Ammonia Nitrogen on Litopenaeus vannamei[J].Journal of Tianjin Agricultural College,2012(3):58-61. |
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| Authors: | WEI Jun-li SUN Jin-sheng Corresponding Author LI Xiang MA Wen-ting GENG Xu-yun XU Yun-xia LI Jing-jing |
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| Institution: | 1(1.Tianjin Center for Control and Prevention of Aquatic Animal Infection Disease,Tianjin 300221,China;2.Tianjin Key Laboratory of Cyto-genetical and Molecular Regulation,Tianjin Normal University,Tianjin 300387,China) |
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| Abstract: | It is studied the acute toxic effects of ammonia nitrogen on two specifications of L.vannamei which mean body weights(MBW) were 0.356 g and 0.036 g,respectively.The concentrations of blood ammonia and activities of alkaline phosphatase(AKP) were analyzed.The results show that variation trend of concentrations of blood ammonia were consistent with the ammonia nitrogen,while the activities of alkaline phosphatase(AKP) were decreasing when the ammonia nitrogen raised.The median lethal concentrations(LC50) of NH3+ on shrimp and the safe concentration(Sc) were also investigated.The LC50 of NH3+ on shrimp with MBW 0.356 g were 2.068 mg/L at 24 h,1.654 mg/L at 48 h and 1.395 mg/L at 96 h,while the LC50 of NH3+ on shrimp with MBW 0.036 g were 1.577 mg/L,1.042 mg/L and 1.012 mg/L,respectively.The Sc was 0.396 9 mg/L and 0.206 5 mg/L to shrimp with MBW 0.356 g and 0.036 g,respectively.It is concluded that the changes of ammonia nitrogen have certain influence on L.vannamei,and the ammonia nitrogen was one of the important factors for the health and the survival of L.vannamei. |
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| Keywords: | Litopenaeus vannamei alkaline phosphatase blood ammonia free nitrogen the median lethal concentration the safe concentration |
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