| 百合上黄瓜花叶病毒的一步RT-PCR检测 |
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| 引用本文： | 王丽花,瞿素萍,杨秀梅,陆琳,吴学尉,王继华.百合上黄瓜花叶病毒的一步RT-PCR检测[J].天津农学院学报,2007,14(4):9-12. |
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| 作者姓名： | 王丽花 瞿素萍 杨秀梅 陆琳 吴学尉 王继华 |
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| 作者单位： | 1. 云南省农业科学院农业部花卉产品质量监督检验测试中心,昆明,650205
2. 云南省农业科学院花卉研究所,昆明,650205 |
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| 摘 要： | 根据黄瓜花叶病毒的外壳蛋白基因序列设计、合成引物,采用Trizol快速提取百合感病组织和健康组织的总RNA,运用RT-PCR两步法和一步法都可从感病组织中扩增出与预期的335 bp大小一致的目标片段,而健康组织无此扩增产物。两种方法相比,一步RT-PCR法特异性强、灵敏度高、省时经济、程序简单,达到了快速检测的目的。
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| 关 键 词： | 百合 一步RT-PCR 检测 |
| 文章编号： | 1008-5394(2007)04-0009-04 |
| 修稿时间： | 2007年9月27日 |
Detection of Cucumber Mosaic Virus on Lily by One-step RT-PCR |
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| WANG Li-hua,QU Su-ping,YANG Xiu-mei,LU Lin,WU Xue-wei,WANG Ji-hua.Detection of Cucumber Mosaic Virus on Lily by One-step RT-PCR[J].Journal of Tianjin Agricultural College,2007,14(4):9-12. |
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| Authors: | WANG Li-hua QU Su-ping YANG Xiu-mei LU Lin WU Xue-wei WANG Ji-hua |
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| Abstract: | A pair of specific primers were designed and synthesized based on the nucleotide sequence of the coat protein gene of cucumber mosaic virus(CMV),and Trizol was adopted to extract the total RNA from the virus-infected tissue and the healthy one.The expected product(335 bp) could be amplified by two-step RT-PCR and one-step RT-PCR from the infected samples,while no amplified products were obtained from the healthy tissue samples.Between the two methods,one-step RT-PCR was more special,sensitive than two-step RT-PCR.It reaches the aim of quick detection. |
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| Keywords: | CMV |
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