大花蕙兰原球茎增殖条件研究

采用原球茎（PLB）进行繁殖是大花蕙兰-种重要的再生方式。本研究以大花蕙兰原球茎为外植体进行研究，比较不同基本培养基（1／3MS、1／2MS、MS）、有机添加物（椰乳、香蕉泥、苹果汁）、活性炭和植物激素（6-BA、NAA）等因子对大花蕙兰原球茎增殖效果的影响。结果表明：1／2MS为基本培养基有利于PLB的增殖；有机添加物对PLB的增殖也有影响，其中，以椰乳的增殖效果最好，其次为香蕉泥、苹果汁；1．5g·L。活性炭可以有效减少褐变的发生；低水平的NAA（O．2mg·L-1）和较高水平的6-BA（1．0mg·L-1）对大花蕙兰PLB的增殖具有显著的促进作用。

Study on PLB Multiplication of Cymbidium hybridium

The effects of different basic culture media （1/3 MS, 1/2 MS, MS）, different organic compounds （coconut milk, banana and apple） and plant hormones （6-BA, NAA） on the propagation of PLB of Cymbidium hybridium were studied by Cymbidium hybridium PLB. The results indicate that 1/2 MS was the optimum basic medium for the propagation of PLB. Organic compounds all have influences on the multiplication of PLB, the promoting effects of coconut milk added into the basic media were better than banana mud and apple juice. 1.5 g ·L-1 activated carbon could absorb phenols effectively, which inhibited the PLB browning. The concentration of 6-BA and NAA could also affect the multiplication of PLB. The lower level of NAA （0.2 mg·L-1 ） with the higher level of 6-BA （ 1.0 mg·L-1） could have significant roles on the promotion of the multiplication of PLB.