| 培养条件对大肠杆菌产L-天冬酰胺酶的影响 |
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| 引用本文： | 范志华,梁鹏,樊秀花,张爱琳,陈一江.培养条件对大肠杆菌产L-天冬酰胺酶的影响[J].天津农学院学报,2008,15(4):29-31. |
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| 作者姓名： | 范志华 梁鹏 樊秀花 张爱琳 陈一江 |
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| 作者单位： | 天津农学院食品科学系,天津,300384 |
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| 基金项目： | 天津农学院科学研究发展基金"大肠杆菌产L-广天冬酰胺酶的研究"
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| 摘 要： | 研究了培养温度、培养时间、初始pH值和摇床转速等培养条件对所选大肠杆菌（E.coli No TN1.5885）产L-天冬酰胺酶的影响。实验结果表明，该菌株进行摇瓶培养的最适培养条件为37℃下培养16h，初始pH值在7.0-7.5间，摇床转速约为300r／min。其比酶活力约为406U／g，比优化前大约提高了20％。
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| 关 键 词： | 培养条件 L-天冬酰胺酶 大肠杆菌 |
Influences of Incubating Conditions on E.coli Accumulating L-asparaginase |
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| FAN Zhi-hua,LIANG Peng,FAN Xiu-hua,ZHANG Ai-lin,CHEN Yi-jiang.Influences of Incubating Conditions on E.coli Accumulating L-asparaginase[J].Journal of Tianjin Agricultural College,2008,15(4):29-31. |
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| Authors: | FAN Zhi-hua LIANG Peng FAN Xiu-hua ZHANG Ai-lin CHEN Yi-jiang |
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| Institution: | (Department of Food Science, Tianjin Agricultural University, Tianjin 300384, China) |
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| Abstract: | The influences of incubating conditions, including incubating temperature, incubating time, initial pH value and rotating speed of shaking table on E.coli （No TN1.5885） accumulating L-asparaginase were studied. The results show that the specific activity of L-asparaginase reached 406 U/g, 20% higher than that before optimization under the optimum conditions of incubating temperature 37℃, incubating time 16 h, the initial pH value between 7.0-7.5 and the rotating speed of shaking table 300 r/min. |
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| Keywords: | incubating conditions L-asparaginase E coli |
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