诺丽营养器官内的总黄酮含量及消长规律

利用紫外分光光度法对诺丽(Noni)营养器官及枝叶不同生长阶段中总黄酮含量的消长规律进行研究,以探索诺丽营养器官内总黄酮的动态积累,为生产中确定适宜的采收期及其采收部位提供依据。结果表明:(1)该方法测定诺丽中总黄酮含量重复性好,稳定性强,加标回收率达99.66%,测试结果稳定可靠;(2)诺丽各器官总黄酮的含量顺序为:90 d叶270 d叶30 d叶毛状根210 d叶150 d叶侧根330 d叶主根嫩枝老枝2年生枝多年生枝1年生枝。结果表明,对诺丽中总黄酮的开发利用应重点选择叶片,采摘生长270 d的叶入药,有利于植株生长,并可提高诺丽资源的综合利用率。

Changes of Total Flavonoids Content in Vegetative Organs of Morinda citrifolia (Noni) in Different Growth Periods

Ultraviolet spectrophotometry was used to study the dynamic changes of total flavonoids in vegetative organs of Noni （Morinda citrifolia Linn.） at different growth stages in a view to determine the appropriate time and organs for harvesting.This method was good in repeatability and high in precision to determine the total flavonoids in Noni.The recovery rate was 99.66％ and the testing result was reliable.The flavonoids content of Noni in different organs varied in the order of 90 days old leaves ＞ 270 days old leaves ＞ 30 days old leaves ＞ hairy roots ＞ 210 days old leaves ＞ 150 days old leaves ＞ lateral roots ＞ 330 days old leaves ＞ taproots ＞ twigs ＞ old branches ＞ biennial branches ＞ perennial branches ＞ annual branches.This indicated that Noni leaves were the priority organ for extraction of total flavonoids in Noni.Considering the plant growth and leaf utilization,it is best to select 270 days old leaves of Noni as the medicinal material to extract total flavonoids.