苹果菠萝的组织培养与快速繁殖

以苹果菠萝的顶芽和吸芽为外植体,接种于MS+6-BA 3 mg·L-1+蔗糖30 g·L-1的培养基上,30 d后腋芽萌发;再将新芽切割,接种于MS+6-BA 2 mg·L-1+NAA 0.05 mg·L-1+蔗糖30 g·L-1的培养基上,30~40 d后形成丛生芽,每30~40 d继代1次,可增殖3~5倍;把丛生芽分割成单株并接种于MS+6-BA 1 mg·L-1+AD 3 mg·L-1+蔗糖30 g·L-1培养基上,进行壮苗培养,25~30 d后再转接种于1/2MS+NAA 0.5 mg·L-1+香蕉汁30 g·L-1+蔗糖30 g·L-1培养基上,30 d后可形成完整的根系;小植株移栽成活率可达95%。

Tissue Culture and Rapid Propagation of Ananas comosus ‘Apple’

The apical buds and suckers of pineapple cultivar ‘ Apple’ （Ananas comosus ‘ Apple’） were inoculated onto MS medium containing 6-BA 3 mg · L^-1 and 30 g · L^-1 sucrose for inducing shoots.The shoots were bourgeoned after cultured for 30 d.They were then transferred onto MS medium supplemented with 6-BA 2 mg · L^-1,NAA 0.05 mg· L^-1 and 30 g · L^-1 sucrose for inducing cluster buds,and the cluster buds were produced after 30-40 d of culture.The proliferation ratio of the cluster buds was about 3-5 per month.The cluster buds were cultured on MS medium containing 6-BA 1 mg · L^-1,AD 3 mg · L^-1 and 30 g · L^-1 sucrose for about 25-30 d to produce strong shoots.These shoots were transferred onto 1/2 MS rooting medium containing NAA 0.50 mg · L^-1,30 g · L^-1 banana juice and 30 g · L^-1 sucrose,and the roots were induced to strike after about 30 d of culture.The rooted plantlets had about 95% of survival rate when transferred onto the soil.