| 多脊椎蒙古羊Hoxc8 exon-1和exon-2的序列分析 |
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| 引用本文： | 陈琦,赵静,张立岭,马月辉.多脊椎蒙古羊Hoxc8 exon-1和exon-2的序列分析[J].热带生物学报,2011(2):157-163. |
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| 作者姓名： | 陈琦 赵静 张立岭 马月辉 |
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| 作者单位： | [1]内蒙古医学院分子生物学研究中心,内蒙古呼和浩特010059 [2]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018 [3]海南大学农学院。海南海口570228 ,内蒙古呼和浩特010018 [4]中国农科院畜牧所,北京100193 |
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| 基金项目： | 国家“十一五”科技支撑项目（2006BDA13B08）; 国家自然科学基金项目（30960245） |
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| 摘 要： | 参考牛的Hoxc8基因序列设计引物,扩增正常蒙古羊（胸椎数13）和多脊椎蒙古羊（胸椎数14）Hoxc8的exon-1和exon-2基因,对得到的序列进行生物信息学分析。结果表明,经序列比对二者的DNA序列,除两侧个别碱基有差异外,中间序列完全一致。蒙古羊Hoxc8的exon-1和exon-2序列分别与其他物种进行同源性...
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| 关 键 词： | 多脊椎蒙古羊 序列分析 Hoxc8 exon-1 Hoxc8exon-2 |
Sequence Analysis of Hoxc8 Exon-1 and Exon-2 of Multi-Vertebrae Mongolia Sheep |
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| CHEN Qi,ZHAO Jing,ZHANG Li-ling,MA Yue-hui.Sequence Analysis of Hoxc8 Exon-1 and Exon-2 of Multi-Vertebrae Mongolia Sheep[J].Journal of Tropical Biology,2011(2):157-163. |
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| Authors: | CHEN Qi ZHAO Jing ZHANG Li-ling MA Yue-hui |
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| Institution: | 1.Research Center of Molecular Biology,Inner Mongolia Medical College,Hohhot 010059,China; 2.College of Animal Science and medicine,Inner Mongolia Agriculture University,Hohhot 010018,China; 3.College of Agriculture,Hainan University,Haikou,570228,China; 4.Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China) |
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| Abstract: | In our study,according to the Hoxc8 sequence of cow,the specific primers were designed,and the sequences of Hoxc8 exon-1（432 bp）and exon-2（273 bp）of normal and multi-thoracic vertebrae mongolia sheep were obtained（Genebank accession number： EU817489 and FJ905472）.Alignment results of them indicated that the sequences were conformity except a little difference in two sides of sequences.Hoxc8 exon-1 and exon-2 were aligned with other species and the results showed that compared with other mammals（human,dog,mouse,rat and chimpanzee）,the homology were above 96%（exon-1） and 91%（exon-2）;compared with zebra fish,the homology were 75.8% and 74%. |
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| Keywords: | Multi-Vertebrae Mongolia sheep sequences analysis Hoxc8 exon-1 Hoxc8 exon-2 |
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