| AtNUDT8过量表达的拟南芥转基因植株 |
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| 引用本文： | 张秀春,李文彬,夏亦荠,彭明.AtNUDT8过量表达的拟南芥转基因植株[J].热带生物学报,2010,1(1):8-11. |
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| 作者姓名： | 张秀春 李文彬 夏亦荠 彭明 |
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| 作者单位： | 张秀春,彭明(中国热带农业科学院,热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海南,海口,571101;农业部转基因植物及植物用微生物环境安全监督检验测试中心,海南,海口,571101);李文彬,夏亦荠(中国热带农业科学院,热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海南,海口,571101) |
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| 基金项目： | 中国热带农业科学院热带生物技术研究所中央级公益性科研院所基本科研业务费专项资助 |
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| 摘 要： | 从拟南芥基因组中克隆AtNUDT8,并与pCHF3相连构建了植物表达载体pCHFN8,采用农杆菌GV3101介导的渗透法转化野生型拟南芥,通过卡那霉素筛选获得了一批经Northern blot检测证实的过量表达AtNUDT8的转基因拟南芥植株。
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| 关 键 词： | AtNUDT8 过量表达 拟南芥 遗传转化 |
Transgenic Arabidopsis thaliana Plants Over-expressing AtNUDT8 |
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| ZHANG Xiu-chun,LI Wen-bin,XIA Yi-ji,PENG Ming.Transgenic Arabidopsis thaliana Plants Over-expressing AtNUDT8[J].Journal of Tropical Biology,2010,1(1):8-11. |
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| Authors: | ZHANG Xiu-chun LI Wen-bin XIA Yi-ji PENG Ming |
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| Institution: | ZHANG Xiu-chun1,2,LI Wen-bin1,XIA Yi-ji1,PENG Ming1,2 (1. Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agriculture Sciences,Key Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture,Haikou 571101,China,2. Environmental Safety Supervision and Inspection Centre for Genetically Modified Plants and Microorganisms used in Plants,) |
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| Abstract: | AtNUDT8 was cloned by PCR from Arabidopsis thaliana and inserted into pCHF3 to form a recombined expressing vector,pCHFN8. Agrobacterium tumefaciens mediating method was used to transform this vector into wide type A. thaliana. And a batch of transgenic A. thaliana over-expressing AtNUDT8 was obtained through kanamycin screening and confirmed by Northern blot. |
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| Keywords: | AtNUDT8 |
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