蜈蚣蕨孢子萌发对光照和温度的响应

用组织培养法和光学显微镜技术初步研究了蜈蚣蕨孢子萌发对光照和温度的响应.结果表明,蜈蚣蕨孢子是需光孢子,具有明显的光休眠现象；光照是影响孢子萌发的主要环境因子,25℃环境下,光照14h·d的孢子萌发率达(85.4±3.1)％；孢子在同温度下黑暗培养49 d不能萌发,照光后,萌发率可达(81.4±5.6)％；孢子在光下萌发的最适温度为19.41～ 30.41℃,3～5d开始萌发,6～7周萌发完全,温度的升高或下降均降低孢子萌发率.

Responses of Pteris vittata L. to Light and Temperature in Spore Germination

Pteris vittata L. , a fern, was tissue cultured for spore germination under different durations of light and darkness at different temperatures and observed under light microscope. The spore of P. vittata L. is light required and has photodormancy. Light is the major environmental factor that influences spore germination.When the fern was cultured at 25 ℃ under the light of ld hrs/day the spores germinated at a rate of （85.4 ±3.1 ） % while no spore germination occurred at the same temperature at the day 49 in dark culture, but when the latter was transferred to culture under the light condition, the spore germination rate increased to （81.4±5.6） %. The optimal temperature for spore germination ranged from 19.41 ℃ to 30.41 ℃ under the light. The spores began to germinate within 3 - 5 days and their germination were completed after 6 - 7 weeks. The temperature beyond the optimal range reduces the spore germination percentage.